Publications by authors named "Sachiko Yamakoshi"

Bovine primary uterine endometrial epithelial cells (EECs) are not ideal for long-term studies, because primary EECs lose hormone responsiveness quickly, and/or they tend to have a short life span. The aims of this study were to establish immortalized bovine EECs and to characterize these cells following long-term cultures. Immortalized bovine EECs were established by transfecting retroviral vectors encoding human papillomavirus (HPV) E6 and E7, and human telomerase reverse transcriptase (hTERT) genes.

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Splenomegaly is one of the typical symptoms of malaria. However, the pathogenesis of splenic enlargement still remains unclear. Spleen is a major organ for clearance of malaria parasites, but excessive response to the parasites can lead to splenomegaly.

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Article Synopsis
  • The study investigates the roles of specific transcripts related to epithelial-mesenchymal transition (EMT) in bovine conceptuses during critical attachment periods (days 17, 20, and 22).
  • RNA extraction and various analyses revealed significant changes in mRNA levels of EMT-related markers, including N-cadherin, vimentin, and matrix metalloproteinases (MMP2 and MMP9), particularly on day 22, alongside downregulation of the epithelial marker CDH1.
  • Additionally, growth factor receptors relevant to EMT and integrin transcripts were examined, showing an upregulation of several receptors, indicating their potential role in the molecular mechanisms that drive EMT in trophectoderm development.
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