Formate Oxidase (FOx) from Aspergillus oryzae: One Catalyst Enables Diverse H O -Dependent Biocatalytic Oxidation Reactions.

An increasing number of biocatalytic oxidation reactions rely on H O as a clean oxidant. The poor robustness of most enzymes towards H O , however, necessitates more efficient systems for in situ H O generation. In analogy to the well-known formate dehydrogenase to promote NADH-dependent reactions, we here propose employing formate oxidase (FOx) to promote H O -dependent enzymatic oxidation reactions.