Gastric motor dysfunction coincides with the onset of obesity in rats fed with high-fat diet.

Exposure to a high-fat diet (HFD) has been reported to impair central autonomic and enteric neurocircuitries, however, the relevant mechanisms and their time course are inadequately clarified. This study aimed to investigate the effects of HFD consumption through the period of adolescence on gastric motor functions in adulthood. Male Sprague-Dawley rats consumed a regular diet or HFD (60% kcal by fat) from 4 to 12 weeks of age.

Serum Sphingolipidomic Analysis in Acne Vulgaris Patients.

Background: Limited data are available on serum levels of different sphingomyelin (SM) and ceramide (CER) species in acne vulgaris (AV).

Objectives: This study aimed to identify circulating levels of neutral sphingomyelinase activity (N-SMase), ceramide-1-phosphate (C1P), sphingosine-1-phosphate (S1P), C16-C24 SMs and C16-C24 CERs in AV patients and controls.

Material And Methods: Serum was collected from 30 AV patients and 20 age, gender-matched control subjects.

Early postoperative changes of sphingomyelins and ceramides after laparoscopic sleeve gastrectomy.

Background: This study aimed to determine early postoperative changes of serum sphingomyelin (SM) and ceramide (CER) species following laparoscopic sleeve gastrectomy (LSG).

Methods: Twenty obese patients [mean body mass index (BMI) 45,64 ± 6,10 kg/m] underwent LSG and normal weight control patients (mean BMI 31,51 ± 6,21 kg/m) underwent laparoscopic cholecystectomy. Fasting blood samples were collected prior to surgery, at day 1 and day 30 after surgery.

Diclofenac induced gastrointestinal and renal toxicity is alleviated by thymoquinone treatment.

The aim of this study was to investigate whether thymoquinone (TQ) could alleviate diclofenac (DCLF)-induced gastrointestinal and renal toxicity in rats. Diclofenac was administered via intramuscular injection twice daily for 5 days and TQ was given by gavage for the same period. Hematological and biochemical profiles were measured with autoanalyzers while reactive oxygen/nitrogen species (ROS/RNS) generation and total antioxidant capacity (TAC) were assayed by standard kits.

Decreased Serum Levels of Sphingomyelins and Ceramides in Sickle Cell Disease Patients.

Limited data are available on the serum levels of different sphingomyelin (CerPCho) and ceramide (CER) species in sickle-cell disease (SCD). This study was aimed at identifying the levels of C16-C24 CerPCho and C16-C24 CER in serum obtained from SCD patients and controls. Circulating levels of neutral sphingomyelinase (N-SMase) activity, ceramide-1-phosphate (C1P), and sphingosine-1-phosphate (S1P) were also determined.

An interference study of the copper-induced plasma oxidizability test.

Objectives: The aim of this study is to investigate the effect of cristaloid cardioplegic fluid (CCF) and its contents on the susceptibility of plasma to copper-catalyzed lipid peroxidation test.

Design And Methods: The plasma pool was divided into eight groups. Equal volumes of CCF or one of its contents were added to each group of the plasma pool.