Evaluation of cytomorphometric changes in tobacco users and diagnosed oral squamous cell carcinoma individuals.

Aims: To determine the cellular and nuclear area of keratinocytes in smears obtained from the oral mucosa of tobacco users, those with oral squamous cell carcinoma (OSCC), and from normal healthy persons and resolve if any significant difference exists in these three groups.

Materials And Methods: The study group comprised 100 subjects 20 controls, (40 OSCC patients-20 from lesional sites and 20 from nonlesional sites, 20 tobacco smokers and 20 tobacco chewers) in the age group of 25-75 years. Oral mucosal smears obtained by using a cytobrush were stained with Papanicolaou (PAP) stain and using 20X objective in trinocular Olympus model BX53 with Jenoptik scientific grade-dedicated microphotographic camera images were taken.

A qualitative and quantitative analysis of AgNORs in keratocystic odontogenic tumor, unicystic ameloblastoma and multicystic ameloblastoma.

Aims: A comparative evaluation of proliferation activity in unicystic ameloblastoma (UA), multicystic ameloblastoma (MA) and keratocystic odontogenic tumor (KCOT) using silver staining technique.

Settings And Design: In the present study 21 histopathologically confirmed paraffin blocks,7 each of UA, MA and KCOT were selected and stained with silver nitrate.

Materials And Methods: For quantitative analysis, 100 cells were counted at 1000x magnification for AgNORs and the mean value was calculated.

Efficacy of 1.5% dish washing solution and 95% lemon water in substituting perilous xylene as a deparaffinizing agent for routine h and e staining procedure: a short study.

Aim. To assess the efficacy of dish washing solution and diluted lemon water in deparaffinizing sections during conventional hematoxylin and eosin staining technique. Objective.