Chromosomal alterations and mutagen sensitivity in human mucosal cells of the oropharynx and lymphocytes caused by BPDE.

Background: In addition to exogenous risk factors, the development of head and neck cancer is based on genetic alterations and individual mutagen sensitivity. DNA damage caused by xenobiotics is not uniformly distributed over the DNA, as certain chromosomes and genes are more likely to be damaged than others. The DNA damaging effect of xenobiotics and the specific sites of chromosomal changes require further investigation.

Antioxidant effects of quercetin and coenzyme Q10 in mini organ cultures of human nasal mucosa cells.

Background: Oxidative DNA damage is a known risk factor of head and neck cancer. Antioxidants, such as coenzyme Q10 (CoQ10) and quercetin, a member of flavonoids present in red wine and tea, are thought to play a significant role in protecting cells from oxidative stress induced by reactive oxygen species (ROS). The aim of this study was to investigate antioxidant effects of quercetin and CoQ10 on mini organ cultures (MOCs) of human nasal mucosa.

COX-2 inhibitors celecoxib and rofecoxib prevent oxidative DNA fragmentation.

Background: Cyclooxygenase (COX) is the key regulatory enzyme in prostaglandin (PG) synthesis and is up-regulated in many premalignant and malignant lesions. The aim of this study was to investigate the in vitro DNA protective or damaging effects of COX-2 inhibitors using the single-cell gel electrophoresis (Comet) assay.

Materials And Methods: Cells from miniorgan cultures of pharyngeal mucosa from 30 patients were incubated once or five times with the COX-2 inhibitors celecoxib and rofecoxib.

The influence of high doses of vitamin C and zinc on oxidative DNA damage.

Background: The influence of interactions between reactive oxygen species (ROS) and dietary antioxidants and their influence on cancer is not clear. It is believed that this effect is mediated by decreased oxidative damage to DNA. The aim of this study was to further investigate the in vitro DNA protective or damaging effects of dietary antioxidants using the single cell gel electrophoresis (Comet) assay.

Sensitivity to DNA-damage induction and chromosomal alterations in mucosa cells from patients with and without cancer of the oropharynx detected by a combination of Comet assay and fluorescence in situ hybridization.

In addition to exogenous risk factors, the development of head and neck cancer is based on genetic alterations and individual sensitivity to mutagens. The DNA-damaging effect of xenobiotics and the location of chromosomal changes warrant further investigation. The aim of this study was to evaluate variance in structural genetic changes in human epithelia as target cells for head and neck carcinogenesis.