Publications by authors named "Saaya Hatakeyama"
In minimal change nephrotic syndrome, podocyte vesicle transport is enhanced. Adenomatous polyposis coli (APC) anchors microtubules to cell membranes and plays an important role in vesicle transport. To clarify the role of APC in vesicle transport in podocytes, nephrotic syndrome was induced by puromycin amino nucleoside (PAN) injection in mice expressing APC1638T lacking the C-terminal of microtubule-binding site (APC1638T mouse); this was examined in renal tissue changes.
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- The study investigates how blocking V-ATPase with bafilomycin B1 affects glucose production and insulin secretion in type 2 diabetic rats.
- Bafilomycin B1 reduces V-ATPase activity, renal gluconeogenesis enzymes, and cytoplasmic glucose, leading to better insulin secretion and lower glucose levels.
- Overall, inhibiting V-ATPase helps manage plasma glucose levels in type 2 diabetes by impacting renal metabolism and enhancing insulin release.
Vacuolar H-adenosine triphosphatase (ATPase) plays important roles in urinary acid excretion, vesicular acidification to activate enzymes, and the membrane recycling of transporters in the kidney. As acidosis stimulates renal gluconeogenesis, we investigated the effect of blockade of H-ATPase on renal gluconeogenesis in diabetic rats. Diabetes mellitus was induced by a single injection of streptozotocin, and a group of DM rats was treated with bafilomycin B1 intraperitoneally for 8 days.
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- Albumin endocytosis is more pronounced in podocytes of rats with minimal change nephrotic syndrome, indicating altered cellular transport mechanisms.
- Researchers used a rat model to observe this phenomenon after injecting Evans Blue-labeled albumin, finding increased vesicle accumulation in podocytes.
- Analysis showed that proteins involved in vesicle transport, like dynein and myosin, were significantly elevated, suggesting a relationship between these proteins and the enhanced endocytosis of albumin.
Angiotensin receptor blocker telmisartan suppresses renal gluconeogenesis during starvation.
Diabetes Metab Syndr Obes
February 2015
The kidney plays an important role in gluconeogenesis during starvation. To clarify the anti-diabetic action of angiotensin receptor blockers, we examined the effects of telmisartan on the sodium-glucose co-transporters (SGLT) and the pathways of renal gluconeogenesis in streptozotocin-induced diabetes mellitus (DM) rats. At 4 weeks, the DM rats treated with/without telmisartan for 2 weeks and normal control rats were used for the study after a 24-hour fast.
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