Use of benzodiazepines in patients with status epilepticus requiring second-line antiseizure medication treatment.

Background: Status epilepticus (SE) is a life-threatening state that needs rapid and adequate treatment. Benzodiazepines (BZD) are used as a first-line treatment for SE, and if the desired effect is not achieved, second-line antiseizure medications are used.

Objective: To investigate whether the treatment with BZDs is performed adequately in patients with different subtypes of SE requiring second-line ASM treatment and, if not, to identify the factors influencing the suboptimal treatment.

Controlling Raman enhancement in particle-aperture hybrid nanostructures by interlayer spacing.

Here we show how surface-enhanced Raman spectroscopy (SERS) features can be fine-tuned in optically active substrates made of layered materials. To demonstrate this, we used DNA-assisted lithography (DALI) to create substrates with silver bowtie nanoparticle-aperture pairs and then coated the samples with rhodamine 6G (R6G) molecules. By varying the spacing between the aperture and particle layer, we were able to control the strength of the interlayer coupling between the plasmon resonances of the apertures and those of the underlying bowtie particles.

Direct Mass Spectrometric Analysis of Brominated Flame Retardants in Synthetic Polymers.

Brominated flame retardants (BFRs) are persistent organic pollutants that pose a major threat to the environment. In this study, a direct insertion probe (DIP) coupled with atmospheric pressure chemical ionization (APCI) quadrupole time-of-flight mass spectrometry (QTOF-MS) was used to characterize additives, especially BFRs, from solid polymer samples with minimal sample preparation. A temperature-programmed DIP analysis, from 150 to 450 °C within 10 min, was utilized to achieve temporal separation of analytes based on their boiling or degradation temperatures, thereby facilitating their easier identification within a single run.

Perspectives of nature-based tourism-dependent communities on climate change in the Okavango Delta, Botswana.

The intensity and frequency of climate extremes such as heat waves, droughts and extreme rainfall events are projected to rise. This will increase the severity of their impacts across socio-ecological systems. Economic sectors such as nature-based tourism become more vulnerable because of their reliance on climate and natural capital as key resources.

Additive manufacturing of double-sided centimeter-scale optics.

In this article we demonstrate rapid manufacturing of an illumination quality double-sided centimeter-scale optics without any post-processing. Two methods are presented: additive manufacturing (AM) using an optically transparent material with a water-soluble support material (support method), and alternatively, using only the optically transparent material but flipping the optics mid-process (flipping method). The main advantage of the flipping method is that only one material is needed.

Targeting CD33+ Acute Myeloid Leukemia with GLK-33, a Lintuzumab-Auristatin Conjugate with a Wide Therapeutic Window.

CD33 (Siglec-3) is a cell surface receptor expressed in approximately 90% of acute myeloid leukemia (AML) blasts, making it an attractive target for therapy of AML. Although previous CD33-targeting antibody-drug conjugates (ADC) like gemtuzumab ozogamicin (GO, Mylotarg) have shown efficacy in AML treatment, they have suffered from toxicity and narrow therapeutic window. This study aimed to develop a novelADCwith improved tolerability and a wider therapeutic window.

Trastuzumab-MMAU Antibody-Auristatin Conjugates: Valine-Glucoserine Linker with Stabilized Maleimide Conjugation Improves In Vivo Efficacy and Tolerability.

Antibody-drug conjugates (ADC) have shown impressive clinical activity with approval of many agents in hematologic and solid tumors. However, challenges remain with both efficacy and safety of ADCs. This study describes novel trastuzumab-auristatin conjugates with the hydrophilic monomethylauristatin E (MMAE) prodrug MMAU, and optimization of a glycopeptide linker leading to a wider therapeutic window.

Participation in inpatient care from the perspective of family members of palliative care patients.

Background: The participation of family members in inpatient palliative care has been shown to be meaningful; it contributes to the wellbeing of the patient. Even so, little is known about the perspectives of family members regarding their participation in this type of care.

Aim: The aim of this study was to describe participation in inpatient care from the perspective of family members of palliative care patients.

Indications for the use of intravenous second-line antiseizure medications in an emergency room setting.

Background: Second-line iv antiseizure medications (ASMs) are used to treat status epilepticus (SE), but in the emergency room setting, there might be other intended and unintended indications for administration. We wanted to explore these different indications and assess the actual usage of first- and second-line ASMs for SE with reference to other uses, such as for SE mimics.

Methods: In this retrospective study, we searched the electronic patient registry with the following terms: "epilepsy", "SE", and "seizure", during 2015.

Effect of clinical features on antiseizure medication doses in patients with newly diagnosed epilepsy.

Objective: We evaluate the effect of distinct clinical features on anti-seizure medication (ASM) doses in seizure-free and not seizure-free patients aged ≥16 years with new-onset epilepsy.

Materials And Methods: This study included 459 patients with a validated diagnosis of epilepsy. The most prescribed ASMs were oxcarbazepine (OXC; = 307), followed by valproic acid (VPA; = 115), carbamazepine (CBZ; = 81), and lamotrigine (LTG; = 67).

Fluctuating climate and dietary innovation drove ratcheted evolution of proboscidean dental traits.

Identification of the selective forces that shaped adaptive phenotypes generally relies on current habitat and function, but these may differ from the context in which adaptations arose. Moreover, the fixation of adaptive change in a fluctuating environment and the mechanisms of long-term trends are still poorly understood, as is the role of behaviour in triggering these processes. Time series of fossils can provide evidence on these questions, but examples of individual lineages with adequate fossil and proxy data over extended periods are rare.

Fast and Tunable Phosphorescence from Organic Ionic Crystals.

Crystalline diphosphonium iodides [MeR P-spacer-R Me]I with phenylene (1, 2), naphthalene (3, 4), biphenyl (5) and anthracene (6) as aromatic spacers, are photoemissive under ambient conditions. The emission colors (λ values from 550 to 880 nm) and intensities (Φ reaching 0.75) are defined by the composition and substitution geometry of the central conjugated chromophore motif, and the anion-π interactions.

From Terminal to Spiro-Phosphonium Acceptors, Remarkable Moieties to Develop Polyaromatic NIR Dyes.

Phosphonium-based compounds gain attention as promising photofunctional materials. As a contribution to the emerging field, we present a series of donor-acceptor ionic dyes, which were constructed by tailoring phosphonium (A) and extended π-NR (D) fragments to an anthracene framework. The alteration of the π-spacer of electron-donating substituents in species with terminal - PPh Me groups exhibits a long absorption wavelength up to λ =527 nm in dichloromethane and shifted the emission to the near-infrared (NIR) region (λ=805 nm for thienyl aniline donor), although at low quantum yield (Φ<0.

Long-term effectiveness of enzyme replacement therapy in Fabry disease with the p.Arg227Ter variant: Fabry disease in Ostrobothnia (FAST) study.

Fabry disease (FD) is an X chromosome-linked, life-threatening lysosomal disease caused by one of more than 1000 currently known variants in the α-galactosidase A (GLA) gene. The follow-up part of the Fabry Disease in Ostrobothnia (FAST) study reports the long-term effect of enzyme replacement therapy (ERT) on a prospectively collected cohort of 12 patients, 4 males and 8 females, mean age 46 years (SD 16), with the classical variant c.679C > T p.

Prescribed antiseizure medication doses and their relation to defined daily doses for achieving seizure freedom in newly diagnosed patients with epilepsy.

Objectives: To investigate the antiseizure medication (ASM) doses required to achieve seizure freedom and their correlation with the World Health Organization's defined daily doses (DDDs) in patients aged 16 years or older with newly diagnosed epilepsy.

Methods: The study included 459 patients with a validated diagnosis of new-onset epilepsy. Patient records were retrospectively analyzed to determine the ASM doses in patients with or without seizure freedom during follow-up.

Response to subsequent antiseizure medications after first antiseizure medication failure in newly diagnosed epilepsy.

Objective: There is a lack of studies using the International League Against Epilepsy (ILAE) recommendation to define drug-resistant epilepsy (DRE). This study evaluated the seizure freedom rates of substitution or add-on and subsequent antiseizure medication (ASM) therapies using different proposed definitions of DRE or ASM trials in patients with a failed first ASM. We also identified prognostic factors for 1-year seizure freedom.

Evolution of the Family Equidae, Subfamily Equinae, in North, Central and South America, Eurasia and Africa during the Plio-Pleistocene.

Studies of horse evolution arose during the middle of the 19th century, and several hypotheses have been proposed for their taxonomy, paleobiogeography, paleoecology and evolution. The present contribution represents a collaboration of 19 multinational experts with the goal of providing an updated summary of Pliocene and Pleistocene North, Central and South American, Eurasian and African horses. At the present time, we recognize 114 valid species across these continents, plus 4 North African species in need of further investigation.

Aerosol processing technique for the synthesis of mixed-phase copper on carbon catalyst: insights into COadsorption and photocatalytic activity.

In this study, spray pyrolysis; an aerosol processing technique was utilized to produce a mixed-phase copper on carbon (Cu/CuO@C) catalyst. The catalyst production was performed via chemical reduction of copper nitrate by a reducing sugar, i.e.

The global spread of misinformation on spiders.

In the internet era, the digital architecture that keeps us connected and informed may also amplify the spread of misinformation. This problem is gaining global attention, as evidence accumulates that misinformation may interfere with democratic processes and undermine collective responses to environmental and health crises. In an increasingly polluted information ecosystem, understanding the factors underlying the generation and spread of misinformation is becoming a pressing scientific and societal challenge.

Forgetting in visual working memory: Internal noise explains decay of feature representations.

The precision of visual working memory (VWM) representations decreases as time passes. It is often assumed that VWM decay is random and caused by internal noise accumulation. However, forgetting in VWM could occur systematically, such that some features deteriorate more rapidly than others.

Clinical utility of a video/audio-based epilepsy monitoring system Nelli.

Objective: The aim of this study was to evaluate the clinical utility of a semi-automated hybrid video/audio-based epilepsy monitoring system (Nelli®) in a home setting.

Methods: In this retrospective study, 104 consecutive patients underwent Nelli-registration for an average of 29 days at their home. The seizure-related data obtained from the registration were assessed to investigate the utility of the Nelli-registration regarding clinical decision-making.

Raman spectroscopy combined with comprehensive gas chromatography for label-free characterization of plasma-derived extracellular vesicle subpopulations.

Raman spectroscopy together with comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS) was employed to characterize exomere- (<50 nm) and exosome-sized (50-80 nm) EVs isolated from human plasma by the novel on-line immunoaffinity chromatography - asymmetric flow field-flow fractionation method. CD9, CD63, and CD81 EVs were selected to represent general EV subpopulations secreted into plasma, while CD61 EVs represented the specific EV subset derived from platelets. Raman spectroscopy could distinguish EVs from non-EV particles, including apolipoprotein B-100-containing lipoproteins, signifying its potential in EV purity assessment.

An expert-curated global database of online newspaper articles on spiders and spider bites.

Mass media plays an important role in the construction and circulation of risk perception associated with animals. Widely feared groups such as spiders frequently end up in the spotlight of traditional and social media. We compiled an expert-curated global database on the online newspaper coverage of human-spider encounters over the past ten years (2010-2020).