Identification of redox activators for continuous reactivation of glyoxal oxidase from Trametes versicolor in a two-enzyme reaction cascade.

Glyoxal oxidases, belonging to the group of copper radical oxidases (CROs), oxidize aldehydes to carboxylic acids, while reducing O to HO. Their activity on furan derivatives like 5-hydroxymethylfurfural (HMF) makes these enzymes promising biocatalysts for the environmentally friendly synthesis of the bioplastics precursor 2,5-furandicarboxylic acid (FDCA). However, glyoxal oxidases suffer from inactivation, which requires the identification of suitable redox activators for efficient substrate conversion.

Copper-radical oxidases: A diverse group of biocatalysts with distinct properties and a broad range of biotechnological applications.

Copper-radical oxidases (CROs) catalyze the two-electron oxidation of a large number of primary alcohols including carbohydrates, polyols and benzylic alcohols as well as aldehydes and α-hydroxy-carbonyl compounds while reducing molecular oxygen to hydrogen peroxide. Initially, CROs like galactose oxidase and glyoxal oxidase were identified only in fungal secretomes. Since the last decade, their representatives have also been identified in some bacteria.

The challenges of using NAD-dependent formate dehydrogenases for CO conversion.

In recent years, CO reduction and utilization have been proposed as an innovative solution for global warming and the ever-growing energy and raw material demands. In contrast to various classical methods, including chemical, electrochemical, and photochemical methods, enzymatic methods offer a green and sustainable option for CO conversion. In addition, enzymatic hydrogenation of CO into platform chemicals could be used to produce economically useful hydrogen storage materials, making it a win-win strategy.

Evaluation of current diagnostic methods for COVID-19.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent responsible for the coronavirus disease of 2019 (COVID-19), which triggers lung failure, pneumonia, and multi-organ dysfunction. This enveloped, positive sense and single-stranded RNA virus can be transmitted through aerosol droplets, direct and indirect contacts. Thus, SARS-CoV-2 is highly contagious and has reached a pandemic level in a few months.

Combating COVID-19 with tissue engineering: a review.

The ongoing COVID-19 pandemic triggered by SARS-CoV-2 emerged from Wuhan, China, firstly in December 2019, as well spread to almost all around the world rapidly. The main reason why this disease spreads so many people in a short time is that the virus could be transmitted from an infected person to another by infected droplets. The new emergence of diseases usually may affect multiple organs; moreover, this disease is such an example.

Tailoring of recombinant FDH: effect of histidine tag location on solubility and catalytic properties of formate dehydrogenase (CtFDH).

Several protein expression systems can be used to get enzymes in required quantities and study their functions. Incorporating a polyhistidine tag is a beneficial way of getting various enzymes such as FDHs for industrial applications. The NAD dependent formate dehydrogenase from (CtFDH) can be utilized for interconversion of formate to carbon dioxide coupled with the conversion of NAD to NADH.

DMSO tolerant NAD(P)H recycler enzyme from a pathogenic bacterium, PC543: effect of N-/C-terminal His Tag extension on protein solubility and activity.

NAD(P) dependent formate dehydrogenase (FDH) is an oxidoreductase used as a biocatalyst to regenerate NAD(P)H in reductase-mediated chiral synthesis reactions. Solvent stability and the need to reduce NADP to NADPH, due to the high cost of NADPH, are required features in the industrial usage of FDHs. Therefore, we aimed to identify a novel, robust NADP dependent FDH and evaluate the effect of N- and C- terminus His tag extensions on protein solubility and activity.

Discovery of an acidic, thermostable and highly NADP dependent formate dehydrogenase from Lactobacillus buchneri NRRL B-30929.

Objectives: To identify a robust NADP dependent formate dehydrogenase from Lactobacillus buchneri NRRL B-30929 (LbFDH) with unique biochemical properties.

Results: A new NADP dependent formate dehydrogenase gene (fdh) was cloned from genomic DNA of L. buchneri NRRL B-30929.

Investigation of ischemia modified albumin, oxidant and antioxidant markers in acute myocardial infarction.

Introduction: Acute myocardial infarction (AMI) is still one of the most common causes of death worldwide. In recent years, for diagnosis of myocardial ischemia, a new parameter, called ischemia modified albumin (IMA), which is thought to be more advantageous than common methods, has been researched.

Aim: In this study, systematic analysis of parameters considered to be related to myocardial ischemia has been performed, comparing between control and myocardial ischemia groups.

Prevention of cyclophosphamide-induced hemorrhagic cystitis by resveratrol: a comparative experimental study with mesna.

Purpose: Hemorrhagic cystitis (HC) is the most common urotoxic side effect of cyclophosphamide (CP). The aim of this study was to compare the classical efficacy of mesna (2-mercaptoethane sulfonate sodium) with three different doses of resveratrol (RES) on cyclophosphamide-induced HC in rats.

Methods: Forty-six male Sprague-Dawley rats were divided into six groups.