Molecular Detection, Phylogenetic Analysis, and Genetic Diversity of Theileria annulata, Babesia bigemina, and Anaplasma marginale in Cattle in Three Districts of Egypt.

Background: Under the poor hygienic conditions, tick-borne pathogens cause severe economic losses to the cattle industry.

Purpose: The current study investigated the presence of Theileria annulata, Babesia bigemina, and Anaplasma marginale, the most relevant tick-borne pathogens in cattle, in 3 provinces of Egypt utilizing species-specific PCR assays.

Methods: PCR was conducted, on bovine blood specimens, using primers targeting the T.