Anti-double stranded DNA antibodies: A rational diagnostic approach in limited-resource settings.

Context: Anti-double-stranded deoxyribonucleic acid antibodies (dsDNA Abs) are highly specific markers of systemic lupus erythematosus (SLE). Multiple methods are employed for their detection in routine diagnostics.

Objectives: The aim of this study was to evaluate a diagnostic approach for anti-dsDNA Abs using DNA-ELISA and fluorescence test (CLIFT), in combination with antinuclear antibody (ANA) screening.