Decrease in CD4+CD25+FoxP3+ Treg cells after pulmonary resection in the treatment of cavity multidrug-resistant tuberculosis.

Objectives: Immune regulatory mechanisms may limit the immunopathologic condition of infection with Mycobacterium tuberculosis and suppress cellular immune responses in the host. We investigated the CD4(+)CD25(+)FoxP3(+) circulating regulatory T cells (T(reg)) in patients with cavity multidrug-resistant tuberculosis (MDR-TB) before and after surgery.

Methods: We compared the proportion of T(reg) cells in 13 patients with cavity MDR-TB pre- and postoperatively and in 10 healthy control subjects by flow cytometry using three specific markers in peripheral blood lymphocytes: cell-surface CD4 and CD25 expression and intracellular FoxP3 expression.

[Ovary cancer mortality in China 2004 - 2005: results from the Third National Retrospective Sampling Survey of Death Causes].

Objective: To analyze the distribution characteristics and trend of ovary cancer mortality based on results of the Third National Retrospective Sampling Survey of Death Causes.

Methods: Data of National Retrospective Sampling Survey for the years of 2004 - 2005 from 158 counties/cities/districts was used for the settings. A total of 69,690,241 person-year for female between 2004 and 2005 including 23,598,822 person-year in urban and 46,091,419 person-year in rural were respectively included in the study.

[Cervical cancer mortality in 2004 - 2005 and changes during last 30 years in China].

Objective: To analyze the cervical cancer mortality and distribution profiles by areas and population in 2004 - 2005 and the changes in the last 30 years.

Methods: The demographic information and cervical cancer death data came from the Third National Retrospective Sampling Survey of Death Causes which including 158 sampling areas (including 61 cities and 97 villages; 52 in the east region, 49 in the middle region and 57 in the west region) covered 31 province-level regions in the interior of China by multiple stage stratified clustering sampling. The total number of the samples were 142 660 482 person years (72 970 241 person years in male and 69 690 241 person years in female).

[Analysis on the mortality of colorectal and anal cancer in China during 2004 - 2005].

Objective: To describe the mortality of colorectal and anal cancer in the Chinese population during 2004 - 2005.

Methods: Mortality of colorectal and anal cancer from The 3rd National Death Retrospective Sampling Survey (2004 - 2005) were analyzed, with that the total population was 142 660 482 person-year and the number of death cases was 10 586. Crude death rate, age-standardized death rate by Chinese standard population (CASR) and world standard population (WASR), the constitute proportion to all cancer deaths and rank of cancer death were calculated and compared with The 1st (during 1973 - 1975) and The 2nd (during 1990 - 1992) National Death Retrospective Surveys.

[Analysis of the esophageal cancer mortality in 2004 - 2005 and its trends during last 30 years in China].

Objective: To describe the mortality of esophageal cancer (EC) in China during 2004 - 2005, and its trends over past 30 years.

Methods: The Third National Retrospective Sampling Survey of Death Causes in 2004 - 2005 was covered 142 660 482 person years (72 970 241 person years in male, 69 690 241 person years in female; 47 899 806 person years in urban, 94 760 676 person years in rural). All death records of EC cases were selected.

[Analysis of liver cancer mortality in the national retrospective sampling survey of death causes in China, 2004 - 2005].

Objective: To analyze the patterns and changes of liver cancer mortality in China.

Methods: Data of national retrospective sampling survey for the years of 2004 - 2005 from 158 counties/cities/districts was used for the settings, covering a 2-year's population of 142 660 482 person years, in which 47 899 806 in urban areas, and 94 760 676 in rural areas. Furthermore, the areas of Eastern, Central, and Western were divided into the subsets, with populations of 52 556 694, 49 781 225 and 40 322 563 person years, respectively.

[An analysis of lung cancer mortality in China, 2004 - 2005].

Objective: To analyze the mortality distribution characteristics and epidemiological trend of lung cancer in 2004 - 2005 in China.

Methods: A total of 142 660 482 person years (72 970 241 person years in male, 69 690 241 person years in female) was covered in the Third National Retrospective Sampling Survey of Death Causes in China, 2004 - 2005. All death records of cancer of trachea, bronchus and lung were extracted.

[Geographical distribution of cancer mortality in China, 2004-2005].

Objective: To describe geographical distribution and its transition of mortality of cancers in China.

Methods: The information of 2 513 949 310 person years were collected in 1973-1975 and 142 660 482 person years in 2004-2005 respectively. Being standardizing the death rates of these two survey with 2000 national census population, the changes of mortality of main cancers was observed and the geographic distribution of cancers in 2004-2005 was analyzed.

Astragalus polysaccharides regulate T cell-mediated immunity via CD11c(high)CD45RB(low) DCs in vitro.

Ethnopharmacological Relevance: Astragalus polysaccharides (APS) isolated from one of the Chinese herbs, Astragalus mongholicus, are known to have a variety of immunomodulatory activities. However, it is not yet clear whether APS can induce the activation and differentiation of dendritic cells (DCs) and subsequently activate T cells.

Aim Of The Study: This study was carried out to investigate the effect of APS on the differentiation of splenic DCs and its influence on T cell-mediated immunity through interleukin (IL)-12-producing CD11c(high)CD45RB(low) DCs in vitro.

PPLook: an automated data mining tool for protein-protein interaction.

Background: Extracting and visualizing of protein-protein interaction (PPI) from text literatures are a meaningful topic in protein science. It assists the identification of interactions among proteins. There is a lack of tools to extract PPI, visualize and classify the results.

[Detecting fire smoke based on the multispectral image].

Smoke detection is very important for preventing forest-fire in the fire early process. Because the traditional technologies based on video and image processing are easily affected by the background dynamic information, three limitations exist in these technologies, i. e.

Inhibition of methicillin-resistant Staphylococcus aureus by the compound Qingre granules.

Background: The infection rate of methicillin-resistant Staphylococcus aureus (MRSA) is increasing yearly due to the overprescription of antibiotics. Traditional Chinese compound medicines are less inclined to induce bacterial resistance in the clinical setting because of their multi-acting mechanisms. However, most current research is limited to bacteriostasis in vitro using single extracts or formulations.

[Epidemiological study on the prevalence of cervical intraepithelial neoplasia among married women aged 25-54 years in Beijing between 2007-2008].

Objective: To investigate prevalence of cervical intraepithelial neoplasia (CIN) among the married women aged 25-54 years in Beijing.

Methods: With method of cross sectional survey, 6339 married women at age of 25 to 54 years were selected randomly in 137 communities of 12 districts or counties in Beijing from March 2007 to September 2008. The cervical smear cytology and high-risk human papillomavirus (HPV) infection of cervical secretion were detected.

Enhanced cholinergic transmission promotes recall in honeybees.

The involvement of the cholinergic system in learning and memory in honeybees has been well established using olfactory conditioning. We examined the effect of Methyl Parathion (MeP), an acetylcholinesterase inhibitor of the organo-phosphate family, on the learning and recall of visual and olfactory discrimination tasks in honeybees. One of our expectations was to observe the effects induced by both the nicotinic and muscarinic systems, as the blocking of acetylcholinesterase should induce an increase in the activity of both systems.

[The intervention effects of different drugs on dendritic cells in acute pancreatitis in mouse].

Objective: To study the immunologic mechanism in pathogenesis of the acute pancreatitis (AP) and the intervention effects of sandostatin and magnolol.

Methods: Ninety BALB/c mice were divided into negative control group, caerulein-induced model group, sandostatin-treatment group, magnolol-treatment group, combined sandostatin- and magnolol-treatment group by means of random number table, with 18 mice in each group. AP model was reproduced by seven intraperitoneal injections of caerulein at an interval of 1 hour.

Naturally existing CD11c(low)CD45RB(high) dendritic cells protect mice from acute severe inflammatory response induced by thermal injury.

Despite a good understanding of the process that initiates and promotes host inflammation induced by acute injury, little is known about the host immune cells responsible for the inhibition of inflammatory response to thermal injury. The aim of this study was to investigate the potential effect of naturally existing CD11c(low)CD45RB(high) dendritic cells (CD11c(low)CD45RB(high) DCs) on acute severe inflammatory response and mortality rate in burned mice. Changes in the percentage of distinct subsets of splenic DCs and production of cytokines (IL-6, TNF-α, CCL-2) as well as CC chemokine (CCL)-2 were measured by FACS at various time points.

[Analysis of correlation factors for cervical intraepithelial neoplasia in married women in Beijing, China].

Objective: To investigate the characteristic of precancerous conditions of cervical intraepithelial neoplasia (CIN) and its related high-risk factors among the married women in Beijing, China.

Methods: Based upon the method of cross-sectional survey, 6339 married women at reproductive age from 25 to 54 years old were selected randomly in a total of 137 communities of 12 districts or counties in Beijing from March 2007 to September 2008. The interview was carried out with unified questionnaires and gynecological examinations.

A method of tumor classification based on wavelet packet transforms and neighborhood rough set.

Tumor classification is an important application domain of gene expression data. Because of its characteristics of high dimensionality and small sample size (SSS), and a great number of redundant genes not related to tumor phenotypes, various feature extraction or gene selection methods have been applied to gene expression data analysis. Wavelet packet transforms (WPT) and neighborhood rough sets (NRS) are effective tools to extract and select features.

[Study on cancer incidence through the cancer registry program in 11 cities and counties, China].

Objective: Aim of this paper was to explore the trend and characteristics of cancer incidence in 11 areas (5 cities and 6 counties) in China.

Methods: Data from cancer registries during 1988 to 2002 collected from the 11 cancer registry points were used to analyze the trends and characteristics of cancer incidence rates.

Results: There were 695 050 newly developed cancer cases in this study.

Changes in lymphocyte subsets in the peripheral blood of patients with active pulmonary tuberculosis.

The aim of this study was to determine the percentage of lymphocyte subsets in peripheral blood in patients with active tuberculosis. A total of 21 patients with active tuberculosis and 15 healthy volunteers were included in the study. T-lymphocyte subsets, B-lymphocytes (CD19(+) cells), natural killer (NK) cells and cells positive for costimulatory molecules CD28 and CD152 were evaluated using flow cytometry.

[Tissue engineering of vascular graft from decellularized arterial matrix and mesenchymal stem cells.].

Objective: To investigate the method of constructing small-diameter vascular grafts from xenogenic decellularized arterial matrices and mesenchymal stem cells (MSCs).

Methods: Porcine iliac arteries were decellularized by detergent and trypsin treatment. Histology, mechanical strength and porosity experiments were performed to evaluate the properties of decellularized matrices.

[Correlation of vasculogenic mimicry with clinicopathologic features and prognosis of ovarian carcinoma].

Objective: To explore the existence of vasculogenic mimicry (VM) in ovarian carcinoma and its correlationship with the clinicopathologic features and prognosis of the tumor.

Methods: A total of 84 ovarian carcinoma cases were collected with complete clinical and prognostic data. CD31 immunohistochemistry and PAS special stain were used to investigate VM in the tumor tissue.

Changes in intestinal mucosal immune barrier in rats with endotoxemia.

Aim: To investigate the dysfunction of the immunological barrier of the intestinal mucosa during endotoxemia and to elucidate the potential mechanism of this dysfunction.

Methods: Male Wistar rats were randomly distributed into two groups: control group and lipopolysaccharide (LPS) group. Endotoxemia was induced by a single caudal venous injection of LPS.