Cloning and characterization of cytochrome c oxidase subunit I (COXI) in Gobiocypris rarus.

In study of gene expression profile in cloned embryos which derived from D. rerio embryonic nuclei and G. rarus enucleated eggs, cytochrome c oxidase subunit I (COXI) of G.

Neurotoxicity of nitrous oxide: multimodal evoked potentials in an abuser.

Introduction: Nitrous oxide (N2O) damages the nervous system of chronic abusers. Multimodal evoked potentials (EPs) can help document the electrophysiological abnormalities of N2O abusers and its distribution in the nervous system.

Case Report: A 41-year-old male N2O abuser had used N2O (4-5 cans/per day, about 2000 ml/can) for more than 10 years.

[Protective effects of ginaton against ischemia-reperfusion injury on the autograft after lung autotransplantation: experiment with rabbits].

Objective: To investigate the effects of ginaton against ischemia-reperfusion injury on the autograft after lung autotransplantation.

Methods: Models of lung autotransplantation were established in 18 New Zealand rabbits were established. The 18 rabbits were randomly divided into 3 equal groups: group of simple ischemia-reperfusion (group I/R), undergoing ischemia by blocking the left pulmonary artery for 2 h and then re-perfusion for 90 min; group with perfusion of low potassium dextran solution (group LPD), undergoing perfusion of LSD solution before ischemia; and group with treatment of ginaton (group LPD + E), undergoing intravenous injection of ginaton 15 min before ischemia.

[Construction of genomic full-length cDNA of SARS coronavirus BJ01 strain and identification of its biological characteristics].

Severe acute respiratory syndrome (SARS) is an important emerging infectious disease which caused by SARS coronavirus (SARS-CoV), and the study of its pathogenesis is needed for the treatment and prevention of this disease. To study the pathogenesis of SARS-CoV using reverse genetics technology, by in vitro ligation using 7 contiguous cDNAs that span the entire genome of the SARS-CoV BJ01 strain, a genomic full-length cDNA was assembled, then using T7 RiboMAX Large Scale RNA Production Systems with the genomic full-length cDNA as template, the RNA transcript was attained. The typical SARS-CoV-resulted cell pathogenic effects were observed when RNA transcript was electroporated into Vero E6 cells.

Circulating preprandial ghrelin to obestatin ratio is increased in human obesity.

Context: Obestatin, a sibling of ghrelin derived from preproghrelin, opposes ghrelin's effects on food intake. Plasma obestatin profiles in relation to ghrelin have not been fully investigated in human obesity.

Objective: We hypothesize that obesity might present with imbalance of circulating ghrelin and obestatin levels.

Nursing care quality: process and outcome relationships.

The relationships between variables in a proposed model for the quality of nursing care provided in the home were examined using a cross-sectional correlational design. Sixty patients discharged from a home-care agency were interviewed in their homes using instruments to measure the model's 7 variables. It was proposed that nursing care provided is related to 3 aspects of the interpersonal process--affective support, health information adequacy, and decisional control--and that these 3 variables are related to 3 outcomes--adherence, symptoms, and well-being.

Anti-Bcl-2 family members, zfBcl-x(L) and zfMcl-1a, prevent cytochrome c release from cells undergoing betanodavirus-induced secondary necrotic cell death.

Nervous necrosis virus (NNV)-induced, host cell apoptosis mediates secondary necrosis by an ill-understood process. In this study, redspotted grouper nervous necrosis virus (RGNNV) is shown to induce mitochondria-mediated necrotic cell death in GL-av cells (fish cells) via cytochrome c release, and anti-apoptotic proteins are shown to protect these cells from death. Western blots revealed that cytochrome c release coincided with disruption of mitochondrial ultrastructure and preceded necrosis, but did not correlate with caspases activation.

Ethnic differences in the frequency of subtypes of childhood acute lymphoblastic leukemia: results of the Malaysia-Singapore Leukemia Study Group.

Childhood acute lymphoblastic leukemia (ALL) is clinically heterogeneous with prognostically and biologically distinct subtypes. Although racial differences in frequency of different types of childhood ALL have been reported, many are confounded by selected or limited population samples. The Malaysia-Singapore (MA-SPORE) Leukemia Study Group provided a unique platform for the study of the frequency of major subgroups of childhood ALL in a large cohort of unselected multiethnic Asian children.

Primary small cell carcinoma of the pancreas with an unusual sonographic appearance.

We present a case of primary pancreatic small cell carcinoma with an unusual sonographic feature. A 75-year-old woman presented with poor appetite and weight loss. Abdominal sonographic examination revealed a diffusely enlarged pancreas with relative increased echogenicity and smooth contour.

[EQCM and in situ FTIR studies on the adsorption and oxidation of 1-butanol at a platinum electrode in alkaline media].

The adsorption and oxidation of 1-butanol in alkaline media on a platinum electrode were investigated mainly by EQCM and in situ FTIR spectroscopy. The experimental results demonstrate that the electrooxidation of 1-butanol is closely relative to solution acidity. Since no chemically adsorbed species, such as CO, were evidenced by in situ FTIR spectroscopy, the adsorption of 1-butanol or its dissociative products on Pt surface is suggested by EQCM and CV data.

Recurrent primary thunderclap headache and benign CNS angiopathy: spectra of the same disorder?

Objectives: To investigate the clinical pictures of patients with recurrent thunderclap headaches of unknown etiology and to field-test two relevant International Classification of Headache Disorders, 2nd edition (ICHD-II) criteria, i.e., primary thunderclap headache (Code 4.

[Listeria-induced host cellular actin cytoskeleton rearrangement and phospholipase D].

Either phagocytosis of macrophage to pathogen or pathogen-induced invasion into non-professional phagocytes, such as epithelial cells, require actin cytoskeletal rearrangements and remodeling of the plasma membrane, which are regulated precisely by monogeric GTPase and the correlated proteins. As a key signaling molecule in the cell, phosphotidicphospholipase D (PLD) regulates or interacts directly with cellular actin cytoskeleton rearrangement. Phospholipase D plays an important role in FcgammaRI and C-reactive protein-mediated phagocytosis and phosphorylated cofilin, a ADF (actin depolymerizing factor) is able to bind to phospholipase D and stimulate it; meanwhile, the Listeria-induced actin cytoskeleton rearrangement during the infection is controlled by the phosphorylation of cofilin.

Functional advantage of NPC-related V-val subtype of Epstein-Barr virus nuclear antigen 1 compared with prototype in epithelial cell line.

Epstein-Barr virus (EBV) is closely associated with nasopharyngeal carcinoma (NPC) and the viral nuclear antigen 1 (EBNA1) plays a crucial role in viral latency. Three EBNA1 subtypes, P-ala, V-thr and V-val have been detected from healthy carriers in Guangzhou area. A close relation of V-val EBNA1 with NPC was suggested by its preference to infect NPC cells.

Comparison of sensitivity and specificity in three commercial foot-and-mouth disease virus non-structural protein ELISA kits with swine sera in Taiwan.

Three commercialized ELISA kits for the detection of antibodies to the non-structural proteins (NSPs) of FMD virus were compared, using sera from uninfected, vaccinated, challenged and naturally infected pigs. The kinetics of the antibody response to NSPs was compared on sequential serum samples in swine from challenge studies and outbreaks. The results showed that ELISA A (UBI) and ELISA B (CEDI) had better sensitivity than that of the 3ABC recombinant protein-based ELISA C (Chekit).

[Short-term curative efficacy of cytokine-induced killer cells combined micro-invasive treatments on hepatocellular carcinoma].

Background & Objective: Recently, micro-invasive treatments showed well application prospective in treating primary hepatocellular carcinoma (HCC), while tumor immunotherapy is a hot topic in tumor treatment. This study was to investigate the efficacy of cytokine-induced killer cells (CIK) combined micro-invasive treatments (transcatheter arterial chemoembolization and radiofrequency ablation) on the recurrence of HCC.

Methods: A total of 85 HCC patients without active lesions and metastasis, which were displayed by imaging examination after transcatheter arterial chemoembolization and radiofrequency ablation therapy, were divided randomly into 2 groups: 45 in study group, and 40 in control group.

Epiploic appendagitis of the rectosigmoid junction: demonstration with transrectal sonography.

A 24-year-old man presented with lower abdominal pain that had been present for 18 hours. Physical examination revealed mild tenderness in the lower abdomen. Digital rectal examination revealed a tender point approximately 9 cm above the anal verge.

Effect of Simotang oral liquid on anal exhaust in patients after abdominal gynecological operation.

Objective: To study the effect of Simotang oral liquid and glycerin enema on the patients' bowel sound (BS) restoration and anal exhaust after abdominal gynecological operation.

Method: Ninety patients with benign tumor who had undergone gynecological operation were randomly divided into the Simotang group, treated after operation with Simotang oral liquid; the enema group, treated with glycerin enema, and the control group, non-treated. The restoration time of BS and anal exhaust were observed.

A new high-nitrogen compound [Mn(ATZ)(H2O)4] x 2H2O: synthesis and characterization.

A new high-nitrogen compound [Mn(ATZ)(H(2)O)(4)] x 2H(2)O (ATZ=5,5-azotetrazolate) was synthesized. Crystal structure and elemental, IR and thermal analyses were investigated in the present work. It crystallized in triclinic space group P-1 with lattice parameters a=6.

In vitro and in vivo studies of a novel potential anticancer agent of isochaihulactone on human lung cancer A549 cells.

We previously demonstrated that the crude acetone extract of Bupleurum scorzonerifolium (BS-AE) 60 microg/ml has anti-proliferation activity and apoptotic effects on A549 non-small cell lung cancer (NSCLC). A novel lignan, isochaihulactone (4-benzo[1,3]dioxol-5-ylmethyl-3(3,4,5-trimethoxyl-benzylidene)-dihydro-furan-2-one), was isolated from BS-AE and identified from spectral evidence ((1)H NMR, (13)C NMR, IR, and MS) and by comparison with authentic synthetic standards. Isochaihulactone was cytotoxic (IC(50)=10-50 microM) in a variety of human tumor cell lines.

Enhanced viability of a nervous necrosis virus-infected stable cell line over-expressing a fusion product of the zfBcl-xL and green fluorescent protein genes.

Nervous necrosis virus (NNV) infection induces host cell apoptosis by an ill-understood process. We utilized a fusion between enhanced green fluorescent protein (EGFP) and the zfBcl-x(L) gene in GL-av cells to select for zfBcl-x(L) stable cell lines and to assess the effectiveness of the anti-apoptotic protein Bcl-x(L) in circumventing NNV-induced cell death. Stable EGFP and EGFP-Bcl-x(L)-expressing clones were obtained at high purity within 2.

[Acupuncture-serum decreases Ca2+ content in cultured rat myocardial cells].

Objective: To explore whether humoral factors play a role in the mechanisms of acupuncture.

Methods: Primary culture of myocardial cells of neonatal rats were carried out. Five days later, they were labeled by fluorescent molecular probe Fluo-3AM.

Injuries associated with teacher assaults: magnitude, nature, cost, and outcome.

The purpose of this retrospective study was to describe the magnitude, nature, and costs of assault injuries among teachers, a high-risk group. Review of assault cases (N = 341) was based on internal and case management vendor records. The 3-year assault rate was 3.