[The use of eeg discriminant analysis in the diagnosis of schizophrenia].

Aim: There have been no criteria found so far to quantify the wide range of EEG spectral and coherent indicators, which would allow discrimination between schizophrenia disorders and healthy human states. The goal of this research is to find objective EEG-based schizophrenia criteria through a discriminant analysis and to obtain a linear discriminant function (LDF) with sensitivity and specificity of at least 85%.

Material And Methods: The study covered 83 schizophrenia patients and 116 healthy individuals, with similar major characteristics.

[Dichotomous classification of autism spectrum disorders: syndromal and non-syndromal forms].

In foreign literature on research into the etiopathogenesis of autism spectrum disorders (ASDs), the division of this group of diseases into two forms is getting more and more frequent. These two forms are 'syndromal' and 'non-syndromal' forms of autistic disorders. The literature review aims to cover the issues of the dichotomous classification of ASDs based on the genetic and molecular psychiatric views on the etiopathogenesis of this group of diseases.

[The efficacy of sulpiride in patients with anxiety disorders depending on the serum serotonin concentrations].

Objective: The efficacy of sulpiride in patients with anxiety disorders depending on the serum serotonin concentrations was evaluated.

Material And Methods: We examined 132 patients with anxiety disorders. The higher levels of serotonin were found in 81 (61.

[Key enzymes of degradation and angiogenesis as a factors of tumor progression in squamous cell carcinoma of the cervix].

A key role in tumor progression play two processes--the destruction and angiogenesis. Matrix metalloproteases (MMPs) play a leading role during tissue degradation. Tissue collagenase--MMP-1 and MT1-MMP hydrolyze fibrillar collagens, which are the basis of connective tissue matrix, and ensure the development of an invasive process.

[Membrane type 1 matrix metalloproteinase (MT1-MMP) and the regulators of its activity as invasive factors in squamous cell cervical carcinomas].

Membrane type 1 matrix metalloproteinase (MT1MMP) is one of matrix metalloproteinases (MMP), which play а key role in tumor invasion and metastasis. The aim of this study was to elucidate the peculiarities of expression of MT1MMP and endogenous regulators of its activity: the activator - furin and the inhibitor - TIMP-2, as invasive factors of squamous cell cervical carcinomas (SCC). The study was carried out using 11 specimens of SCC and 11 specimens of morphologically normal tissue adjacent to the tumor.

[The effectiveness of BACTEC MGIT 960 system hit analysis of surgery material of patients with tubercular spondylitis].

The article considers the comparison of effectiveness of cultivation of 290 surgery samples from focus of destruction in patients with tubercular spondylitis in liquid medium Middlebrook 7119 with fluorescent detection of growth and two dense egg medium -Levenschtein-Yensen and Finn-II. The sensitivity of methods of inoculation in liquid medium Middlebrook 7H9 and in dense egg mediums has no difference (38.2% and 43.

[Molecular-genetic characteristics of Mycobacterium tuberculosis strains isolated from patients with tuberculous spondylitis].

Aim: Molecular-genetic characteristic of M. tuberculosis strains isolated from operation material of patients with tuberculous spondylitis.

Materials And Methods: 107 strains of M.

[Matrix metalloproteinases (MMP)--MMP-1,-2,-9 and its endogenous activity regulators in transformed by E7 oncogene HPV16 and HPV18 cervical carcinoma cell lines].

Matrix metalloproteinases (MMP) play a key role in development of tumor invasion and metestasis. The purpose of the work is the elucidation of peculiarities of expression of MMP-1, MMP-2, MMP-9 and their activity regulators: plasminogen activator uPA and tissue inhibitors of MMPs - TIMP-1 and TIMP-2 in human cell lines of squoamous cell carcinoma (SCC). Comparative study of MMPs' expression was carried out on cell lines SCC which differed in HPV types (HPV-16 and HPV-18): SiHa, Caski - HPV16, Hela, C4-1 - HPV18).

[Interstitial collagenase, gelatinases A and B and their endogenous inhibitors in squamous cell cervical carcinomas].

Interstitial collagenase and gelatinases are matrix metalloproteinases (MMP), which play the key role in tumor invasion and metastasis. The aim of this study was to elucidate the peculiarities of expression of interstitial collagenase (MMP-1), gelatinases A and B (MMP-2 and MMP-9) and their endogenous tissue inhibitors TIMP-1 and TIMP-2 as invasive factors of squamous cell carcinomas (SCC) of human cervical cancer. The study was carried out using 24 specimens of SCC and 11 specimens of adjacent to tumor morphologically normal tissue.

[Cardiac resynchronization therapy in patients with narrow QRS].

Conduction delay affecting 30-50% of patients with NYHA class III-IV heart failure (HF) mainly results from left bundle branch block and leads to deterioration of cardiac contractility through intra- and interventricular dyssynchrony. Cardiac resynchronization therapy (CRT) has class I recommendation for the treatment of patients with severe systolic HF who have left ventricular ejection fraction less or equal to 35%, QRS duration greater than or equal to 120 ms. Nevertheless some studies have shown that systolic asynchrony is present in 27-43% of HF patients with narrow QRS complexes (defined as <120 ms).

[Physiological significance of amine oxidases and technique for determination of their activity].

The paper describes the physiological effects of various biogenic amines, their occurrence in the body, and degradation. It outlines the present views of diversity of flavin adenine dinucleotide-dependent and semicarbazide-sensitive amine oxidases. Methods for preparation of various tissue extracts and spectrophotometric and radiation techniques for determination of the activity of these enzymes, by using various substrates, are described.

[Endothelial cell changes preceding the formation of intimal erosions in coronary arteries and aorta in atherosclerosis in humans].

The cytological technique was used to study autopsy specimens from 39 patients aged 35-85 years. Above the atherosclerotic strips and plaques there were multiple destruction microfoci (MDMs) of endothelial cells (EC), which were divided into three types. EC MDMs were detected in the aortic intima in 79.

[The implication of Chlamydia pneumoniae in damage to human aortic endotheliocytes in atherosclerosis].

A cytological technique was used to study the impressions of the aortic intima of dead patients. Endothelial cell Chlamydia pneumoniae (CP) was detected in 26 (56.5%) of 46 dead patients; the bacterium was found in the unaffected intact intimal areas in 9 (19.

[Methods for determining matrix metalloproteinase activity].

The paper describes methods for determining the activity of matrix metalloproteinases (MMP), primarily collagenases and gelatinases, by applying natural protein substrates. Reconstructed fluorescein-labeled type I collagen fibrils were used to determine collagenases as a substrate. The zymographic technique in polyacrylamide gel with copolymerized gelatin was employed for the identification and assay of gellatinases.

[Expression of gelatinases A and B and their endogenous regulators in immortal and transformed fibroblasts].

Matrix metalloproteinases (MMP) play a critical role in tumor invasion and metastasis. The aim of this study was to elucidate peculiarity of expression of gelatinases A and B (MMP-2 and MMP-9), membrane type MMP (MT1-MMP) and tissue inhibitor of MMP (TIMP-2) in immortal (IF) and transformed fibroblasts (TF).The study was carried out using embryo rat fibroblasts, sequentially immortalized with the polyomavirus LT gene and transformed with the E7 gene of human papilloma virus (HPV-16).

[Analysis of the heterogenicity of cytological and morphometric indices of the endothelial nuclei of the aorta and pulmonary artery in atherosclerosis].

The authors made a qualitative cytological analysis of the percentage of the heterogenic endothelium and its association with the severity of atherosclerosis. A total of 15,000 endotheliocytes (usual-type, giant uninucleated, binucleated, trinucleated, multinucleated, and horseshoe-shaped nuclei) were studied above different forms of atherosclerotic lesion of the human aorta and pulmonary artery. The material was obtained from 5 deceased patients aged 35 to 85 years.

[Combined use of molecular genetic technologies to identify the Russian citizens died during tsunami in Thailand].

Main stages, methodological aspects and practical results of molecular genetic research to identify the citizens of Russia died during tsunami in Thailand are considered.

NO synthase-dependent increases in extracellular citrulline levels in the nucleus accumbens in an emotional conditioned reflex.

Intracerebral microdialysis/HPLC studies in Sprague-Dawley rats showed that the acquisition and execution of an emotional conditioned reflex was accompanied by an increase in the extracellular citrulline level in the nucleus accumbens; citrulline is a co-product of nitric oxide synthesis. The increase in the citrulline level evoked by execution of this reflex decreased after injection of 7-nitroindazole (0.5 mM), a selective inhibitor of neuronal NO synthase, into the nucleus accumbens, and was completely blocked by injection of N-nitroarginine (0.

Regulation of c-Fos gene expression in the rat olfactory bulb during olfactory learning.

An immunohistochemical method was used to study the expression of transcription factor c-Fos in the mid part of the main olfactory bulbs in 18-day-old rats after training to seek their mothers using an olfactory orientation cue (propionic acid) in a Y-maze. On the next day, rat pups demonstrated a significant preference for the propionic acid odor in behavioral tests, as compared with control animals trained to the skill without the olfactory orientation cue and pups familiarized with the odor without forming any association between it and any kind of reinforcement. The propionic acid odor evoked an insignificant increase in c-Fos expression, predominantly in the granular layer of the dorsomedial area of the olfactory bulb.

[Expression of interstitial collagenase and its endogenous regulators in immortalized and transformed by E7 gene HPV16 fibroblasts].

Matrix metalloproteinases (MMPs) play a critical role in tumor development and invasion. The aim of this study is to elucidate peculiarity of expression of interstitial collagenase (MMP-1) and its endogenous regulators in the process of oncogenic transformation of fibroblasts by E7 gene of HPV16. Papilloma virus type 16 and 18 are aetiological factor of cervical cancer.

[The character of dyslipidemia and myocardial and carotid arterial remodeling in young patients with essential hypertension].

The purpose of the investigation was to study the character of myocardial and vascular revascularization by the example of the carotid artery (CA) in young patients with arterial hypertension in correlation with biochemical and clinical data. Sixty-eight patients were examined. EchoCG and sonography were performed using SONOS 4500 device.

[Study of substrate specificity of a new peptide substrate of endothelin converting enzyme].

The new fluorogenic hexapeptide substrate CMC-Ala-Gly-Gly-Trp-Phe-Arg was used as substrate for endothelin-converting enzyme (ECE), angiotensin-converting enzyme (ACE) and neutral endopeptidase (NEP). The specific inhibitors lisinopril (ACE) and thiorphan (NEP) were used for identification of these enzyme activities,

[Correlations between electrophysiological myocardial remodeling and parameters of free radical oxidation in patients with various forms of ischemic heart disease].

Aim: To investigate potentialities of a novel biochemical test assessing oxidative resistance of plasma as a marker of ischemic damage to the myocardium and to study this marker correlation with high-performance ECG parameters reflecting electrophysiological remodeling of the myocardium in patients with ischemic heart disease (IHD).

Material And Methods: A total of 145 IHD patients entered the trial: 32 patients free of effort angina, 67patients with stable effort angina of functional class (FC) II-III, 56 patients with acute coronary syndrome (ACS) and 32 healthy controls. ACS patients were examined according to the following protocol: stage 1--6-12 hours since the disease onset, stage 2--at the end of the first 24 hours, stage 3--day 5-7 of the disease.

[Regulation of c-Fos expression in the rat olfactory bulbs during olfactory learning].

The distribution of c-Fos-immunopositive neurons was examined in the mitral/tufted and granular cell layers in the medium part of the main olfactory bulbs of 18-day-old rats after they had been trained for propionic acid vapour-guided search for dam in the Y-maze. On the next day these pups exhibited a strong preference for the propionic acid odor as compared to the control pups trained for this task without the odor cue and odor-familiarized pups exposed to propionic acid as a novel neutral stimulus. Exposure to propionic acid produced a moderate activation of c-Fos expression, mainly in the granular layer of the dorsomedial part of the bulb.