Effects of Combined Exposure to Carbon Ions and Protons on the Pool of MCF-7 Breast Cancer Stem Cells In Vitro.

We studied the effects of single and combined action of protons and carbon ions C on the pool of MCF-7 human breast cancer stem cells. Single irradiation with a beam of protons or carbon ions had no significant effects on the relative number of cancer stem cells (CSC). The effects of combined irradiation in a total equieffective dose of 4 Gy depended on the sequence of exposure to ionizing radiations: the relative number of CSC did not change after irradiation with carbon ions and then with protons, but increased in the case of the reverse sequence.

Biological Response of Chinese Hamster B14-150 Cells to Sequential Combined Exposure to Protons and C Ions.

Proton and ion radiation therapy, when used both as single radiation and in mixed radiation mode, have a number of advantages over the conventional γ-therapy that are determined by physical characteristics of accelerated particles. The paper presents the results of an in vitro study of the effectiveness of sequential exposures of Chinese hamster tumor cells B14-150 to proton (p) and C ion beams. We used 4 irradiation schemes differing by the sequence of exposure and the contribution of each radiation to the total dose.

Post-Irradiation Recovery of B14-150 Fibrosarcoma Cells after Combined Irradiation with Low and High Linear Energy Transfer.

The use of radiation with low and high linear energy transfer (LET) in the same treatment regimen is promising in terms of increasing the efficiency and reducing the severity of radiation complications. Here we studied combined effect of protons (LET≈3 keV/μm) and heavy recoils (HR) induced by 14.5 MeV neutrons (LET≈290 keV/μm) on B14-150 fibrosarcoma cells.

Calculation of the Biological Efficiency of the Proton Component from 14.8 MeV Neutron Irradiation in Computational Biology with Help of Video Cards.

Fast neutron therapy, which previously has demonstrated effective results, but along with a large number of complications, can again be considered a promising treatment method in the treatment of cancer. One of the ways of analyzing the relative biological efficiency and accurate biological dose of fast neutrons in body tissues is to improve the algorithms of computational biology and mathematical modeling. A high-performance computing code was written which allows to estimate in real-time mode the biological dose of the proton component from the action of neutron radiation with an energy of 14.

[Strangulation of the appendix in an inguinal hernia sac in children].

Objective: To study the features of the clinic and treatment of children with infringement of the appendix in inguinal hernias in children.

Material And Methods: We observed 12 children with the localization of the appendix in the right inguinal hernia. Four of them had an infringement of the appendix: two with inguinal hernia, two with sliding inguinal and femoral hernias.

Comparison of Predictive Tools on Missense Variants in , , and Genes Associated with Autosomal Recessive Deafness 1A (DFNB1A).

predictive software allows assessing the effect of amino acid substitutions on the structure or function of a protein without conducting functional studies. The accuracy of pathogenicity prediction tools has not been previously assessed for variants associated with autosomal recessive deafness 1A (DFNB1A). Here, we identify tools with the most accurate clinical significance predictions for missense variants of the (Cx26), (Cx30), and (Cx31) connexin genes associated with DFNB1A.

The origin of the "dark" absorption band near 675 nm in the purple bacterial core light-harvesting complex LH1: two-photon measurements of LH1 and its subunit B820.

A comparative two-photon excitation spectroscopic study of the exciton structure of the core antenna complex (LH1) and its subunit B820 was carried out. LH1 and its subunit B820 were isolated from cells of the carotenoid-less mutant G9 of Rhodospirillum rubrum. The measurements were performed by two-photon pump-probe spectroscopy.

Formation of a Subunit Form of the Core Light-Harvesting Complex from Sulfur Purple Bacteria Ectothiorhodospira haloalkaliphila with Different Carotenoid Composition.

B820 subunits from a purple sulfur bacterium Ectothiorhodospira. haloalkaliphila strain ATCC 51935T were obtained by treatment of Carotenoid free LH I-RC complexes of this bacterium with P--octylglu- copyranoside (β-OG). The same complexes with 100% carotenoid content were unable to dissociate to B820 subunits, but disintegrated to monomeric bacteriochlorophyll (BChl) regardless of their carotenoid compo- sition.

[Morphoecological Aspects of Preimaginal Stages of Development of the Parasitic Wasp Minotetrastichus frontalis (Nees, 1834) (Insecta, Hymenoptera, Eulophidae)].

This work is devoted to studying the morphology and biology of preimaginal stages of development of the parasitic wasp Minotetrastichus frontalis. The morphological characteristics of eggs and larvae, the features of interaction of the parasitic wasp with the host (Phyllonorycter issikii), and other biological characteristics were determined. For the species identification of the studied specimens of insects, we performed molecular genetic analysis using a nuclear 28S RNA gene fragment as a molecular marker.

[The audiological analysis in the patients homozygous for the c.-23+1G>A mutation in the GJB2 gene presenting with the loss of hearing in Yakutiya].

In the course of previous investigations carried out in the Republic of Sakha (Yakutiya), we have identified the main molecular-genetic factor responsible for the hereditary impairment of hearing among the indigenous population (mostly the Yakuts).The disease was shown to be attributable to the c.-23+1G>A mutation localized in the splice donor site (exon 1) of the GJB2 (Cx26) gene.

[Formation of 55-kDa Fragments under Impaired Coordination Bonds and Hydrophobic Interactions in Peripheral Light-Harvesting Complexes Isolated from Photosynthetic Purple Bacteria].

Size exclusion chromatography was used to assess the relative size of intact and diphenylamine-treated (DPA, with suppressed carotenoid synthesis) peripheral light-harvesting complexes (LH2 complexes) of the sulfurbacterium Allochromatium minutissimum. Both LH2 complexes were nonamers and had the same elution volume V(e), coinciding with that for the LH2 complex of Rhodoblastus acidophilus (strain 10050). Their molecular mass was 150 kDa.

[Analysis of wheat and rye semidwarfing gene distribution in spring hexaploid triticale (Triticosecale Wittm.) varieties and lines].

A collection of spring hexaploid triticale varieties and promising breeding lines has been examined for the presence of wheat Rht-B1b, Rht-B1e, and Rht8c semidwarfing genes and the rye Hl semidwarfing gene. It was discovered in spring triticale that these semidwarfing genes are represented by only one, the Rht-B1b wheat gene. The presence of this gene is associated with shortening of spring triticale plants by 28 cm on average, which constituted 26% of their initial height.

[On the problem of registration of mortality associated with alcohol consumption among the population in the forensic medical practice].

The objective of the present study was the comparative analysis of the data obtained from the available medical documentation with a view to estimating the effectiveness of taking into account the mortality associated with alcohol consumption. The data subjected to the analysis included the age and the gender of the deceased subjects, complete forensic medical diagnoses from 1607 expert reports and acts of forensic medical expertise of the corpses (form 171/y), and information about the cause of death (item 19) from the relevant medical death certificates (form 106y/08). The mortality associated with alcohol consumption estimated based on the expert reports and acts of forensic medical expertise of the corpses (form 171/y) amounted to 6.

[Breeding dynamic of corvids in urbanized landscapes of the European east].

On the basis of counting and mapping of nests of the rook, hooded crow, and magpie in Kirov in 1975-2012, it was found that, under the conditions of an industrial city, rook colonies are highly unstable. The elimination of the private sector and neglected tree and scrub thickets leads to a reduction in the number of magpies nesting in the city. The nesting abundance of crows is limited by the quarter layout and placement of containers for household waste.

[Alcohol-related mortality in the assessments of hospital unit physicians and pathologists: analysis of accounting medical documents].

Aim: To make a comparative analysis of the data available in the accounting medical documents drawn up at a multidisciplinary hospital on the level and structure of alcohol-related mortality (ARM) and to evaluate the efficiency of its accounting.

Materials And Methods: Accounting medical documents, such as 453 inpatient cards (Form 003/y), 453 postmortem protocols (cards) (Form 013/H-80), and 453 death certificates (Form 106/y-08), were chosen as the basis for the study. The data of the final clinical and postmortem diagnoses in the patients who had died at hospital and their primary cause of death were comparatively analyzed.

[Socio-demographic status of elderly and senile age people died from alcohol-attributable states in Arkhangelsk].

To determine the characteristics of the socio-demographic status of elderly and senile age people died of alcohol-attributable states in Arkhangelsk, data from the medical certificate of death was analyzed. Data of death for persons who died in the city of Arkhangelsk in the period from 1 July 2011 to 30 June 2012 (one year) were copied out. The vast majority of alcohol conditioned deaths among men occurs in middle (working) age, 1/3 of women--over the age of 60 years.

[Hybrid surgery for the treatment of ovarial cysts in newborns].

The aim of this study was to evaluate the effect of hybrid technology (laparoscopy and open surgery) for treatment of ovarian cysts in small babies and infants. Between January 2002 and November 2012, we have performed 11 operation for neonatal ovarian cysts repaired through standard circumumbilical incision (Group I) and 16 operation with using combined hybrid laparoscopic-assisted technique (Group II). The circumumbilical incision utilized at our institution is a classic Bianchi procedure.

[The inborn flat-valgus deformity of the foot in children].

To optimize the diagnosis and treatment for the inborn flat-valgus deformity of the foot (IFVDF), the results of treatment of 78 children, ageing from 1 mo to 12 yrs old, were analyzed. Algorithms of diagnosis and treatment were elaborated. Operative treatment on soft tissues for IFVDF was indicated in the cases of inefficacy or insufficient efficacy of conservative therapy in the age after 1 year old, when a child starts to walk by himself.

[Role of bacteriochlorophyll in stabilization of the structure of the near-central and peripheral light-harvesting complexes from purple photosynthetic bacteria].

Pheophytinization of bacteriochlorophyll (BChl) at low pH was investigated in the core (LH1) and peripheral (LH2) light-harvesting complexes, as well as in the ensemble of the reaction center (RC) with the LH1 complex. The stages in disintegration of the native BChl forms in the LH1 complex and in its ensemble with RC were revealed. They were observed as emergence of the absorption band of monomeric BChl and an increase in its intensity, followed by its transformation into the band of monomeric bacteriopheophytin (BPh) and then into the band of aggregated BPh.

[Spinal muscular atrophy carrier frequency in Ukraine].

Background: Spinal muscular atrophy (SMA) is a common autosomal recessive neuromuscular disorder with the frequency of carriers in a number of ethnical groups ranging from 1/50 to 1/25. However, the prevalence of SMA for population of Ukraine remains to be established.

Methods: For the analysis of deletion in exon 7 SMN1 gene in SYBR Green Real-Time qPCR assay specific for the single nucleotide change in exon 7 (cd 840 C >T) was used.

[Prevention of beer dependence in adolescents in educational institutions].

The authors have systematized approaches to the prevention of beer dependence in adolescents and presented the inner structure of prevention in educational institutions in terms of consecutive steps. The author's methods for adolescence beer dependence diagnosis are described. Different forms of preventive work with adolescences and their parents allowing to combine flexibly the preventive methods with the participation of cross-functional specialists are presented.

[The mortality because of conditions related to alcohol consumption].

The article presents the systematization of data concerning alcohol-depended mortality as a occurrence with complicated structure. It is established that grouping of alcohol-depended mortality on isolated components is conditioned by different nature of unhealthy consumption of alcohol in the development of alcohol-depended conditions as a conditional and/or supporting factor. Among other causation factors article points out the input of pattern of alcohol consumption into the value of risk of development of alcohol-attributive conditions and two-way impact of unhealthy alcohol consumption at the risk of development of particular alcohol-attributive conditions.

[Effectiveness of the medical-psychological rehabilitation of combatants].

To analyze treatment effectiveness, authors examined 199 combatants during the first days and in 1.5 month after their return from combat zones. Stages and content of medical-psychological rehabilitation, including psychopharmacotherapy and psychotherapy, are described.