[Live enteroviral vaccines for the emergency nonspecific prevention of mass respiratory diseases during fall-winter epidemics of influenza and acute respiratory diseases].

The results of the 3-year controlled trials of a new method of nonspecific urgent prophylaxis of influenza and acute respiratory diseases (ADR) by immunization of healthy adults with standard live enterovirus oral vaccines, introduced in 2-3 administrations at intervals of 7-10 days, at the initial stages of autumn and winter epidemics are presented. Observations, carried out in three republics, covered more than 150,000 persons immunized with enterovirus interferonogenic vaccines. A considerable decrease in morbidity rate among the vaccinees was achieved (on the average, by 3.

An experience with application of human leucocyte interferon for cervical cancer treatment.

Interferon was injected intracervically as the first stage of combination treatment in 125 cases of histologically verified squamous Ca in situ and invasive cervical carcinoma. Colpomicroscopic, cytological and histological examination was carried out before and after treatment. The pronounced effect of interferon as an antitumor agent was established.

[Immune structure and rubella incidence in pregnant women in Moscow].

The immune structure of the Moscow City population (mostly, pregnant women) in relation to rubella virus antigen. Specific antihemagglutinins were found in 82%-93% of pregnant women, depending on their age. Examination of sera from 207 pregnant women who had contacts with rubella patients demonstrated clinically manifest rubella verified serologically in 9.

[Biosynthesis and main biological properties of human gamma interferon].

Conditions for and regularities of gamma-interferon production were elucidated. High interferon inducing activity of the known inductors such as concanavalin A, phytohemagglutinin and lentil lectin was asserted. It was shown that aqueous and alcoholic extracts of legumes seeds were also able to induce interferon synthesis in cultures of human peripheral blood leukocytes.

[Experimental coronavirus encephalomyelitis in mice].

A combined comparative virological, morphological, and immunological study of experimental coronavirus encephalomyelitis was carried out in mice in order to elucidate the pathogenetic mechanisms involved in the formation of the foci of lesions in acute and chronic forms of the disease. Intracerebral inoculation of C3H mice with the neurotropic JHM strain of murine hepatitis virus induces a disease with demyelinization foci in the CNS running acute, subacute, or chronic course. This model underlies a concept that demyelinating diseases are caused by viruses producing immunopathologic responses realized via certain histocompatibility loci.

[Effect of regulator cells induced by the influenza virus in adoptive transfer].

The protective effect of humoral and cellular immunity factors on experimental mouse influenza infection was studied in combination with a simultaneous analysis of the functional activity in the regulatory lymphocytes of donor mice. The efficacy of adoptive defense of recipient mice, that is the intensity of immune reactions in their organisms, was found to depend on the concrete functional state of donor mouse transferring cells. The exact time of activation of T-helper cells open certain prospects for the concrete pathogenetically grounded drug therapy.

Study of live recombinant cold-adapted influenza bivalent vaccine of type A for use in children: an epidemiological control trial.

Live cold-adapted recombinant bivalent vaccine of influenza type A was studied in a controlled field trial in 1982-1983 among nearly 30,000 children 3-15 years old. The bivalent vaccine consisted of recombinants 47/25/1 (H1N1) and 47/7/2 (H3N2) of wild-type viruses A/Brazil/11/78 (H1N1) and A/Bangkok/1/79 (H3N2) with cold-adapted donor A/Leningrad/134/47/57 (H2N2). The recombinants which received mutant nonglycoprotein genes from cold-adapted donor did not suppress each other after simultaneous inoculation of children and stimulated antibody response to both strains.

[Experience with clinical use of human leukocyte interferon in cancer of the cervix uteri].

Interferon was injected intracervically as the first stage of combination treatment in 110 cases of histologically-verified Ca in situ and invasive cervical carcinoma. Colpomicroscopic, cytologic and histologic examination was carried before and after treatment. Posttreatment histologic examination failed to detect any tumor cells in 31 patients (28.

[Characteristics of the interaction of the influenza and vesicular stomatitis viruses with the splenocytes of inbred mice].

The reproduction activity of many viruses in cells of human and animal immune system has not been studied sufficiently on the body level. It is especially important to determine exactly the sensitivity of the main triad of the immune system cells to a certain virus from the parameters of its intracellular reproduction. Complete elimination of extracellular influenza A/PR8/34 virus from in vivo pre-infected cells of the immune system of mice revealed no reproduction of this virus.

[Characteristics of the immunological and protective effect in the simultaneous and separate use of live and killed influenza vaccines].

Simultaneous immunization with inactivated and live influenza vaccines is characterized by a more rapid and intensive formation of local protection and humoral immunity persisting for 5 months at a higher level than after separate administration of inactivated and live vaccines. The live vaccine was found to be the major factor enhancing the immune response and local protection in combined immunization. Administration as components of a live bivaccine of influenza serotype A viruses with a different degree of attenuation was not accompanied by higher reactogenicity but led to a sharp decrease of immunogenic activity that indicated incompatibility of such viruses in a bivaccine.

[Changes in the biological properties of influenza virus (HON1) strains virulent for mice when exposed to homologous antibodies].

A decrease in the virulent properties of two influenza virus strains (A/PR/8/34 and A/Mel/35 (HON1) pathogenic for mice was demonstrated in multiple passages in chick embryos in the presence of homologous antibody. The artificially developed avirulent A/PR/8/240 and A/Mel/375 variants had low immunogenic activity and a marked temperature sensitivity to 40 degrees C. Common ts mutations in the genomes of these avirulent variants located in genes 1 and 7 coding for P3 and M proteins were demonstrated.

[Protective effectiveness of the blood sera and immune system cells from mice immunized with live or inactivated influenza virus].

The results of the study of comparative protective role of humoral and cell-mediated immunity factors obtained from inbred donor mice immunized with live or inactivated influenza virus are presented. The superiority of the live virus over the inactivated preparation as the inducer of not only humoral but especially cell-mediated immune response was demonstrated by the effectiveness of passive intranasal protection of the infected mice, by the degree of inhibition of virus reproduction in the lungs of the protected mice, and by the capacity for interferon production by the cells of the immune system of donor mice.