Effect of alpha-tocopherol, pyridoxine and dexpanthenol on the stress in crease of nonesterified fatty acids levels in the brain.

The supposed antistress effect of vitamins-alpha-tocopherol, pyridoxine and dexpanthenol (pantothenic acid precursor)--was followed on the model of nociceptive stress in laboratory rats. The decrease of the stress enhancement of nonesterified fatty acids (NEFA), estimated in the brain cortex, hypothalamus and the brain stem, was taken for the indicator of the antistress effect. Nonesterified fatty acids were determined with the help of gas chromatography following the separation performed by thin layer chromatographic method.

The labilizing and stabilizing effect of physostigmine on CNS.

The authors observed biphasic changes of dopamine, norepinephrine and serotonin in the rat brain stem after a single application of physostigmine (1 mg/kg of body weight). The results confirm the hypothesis of "labilization and stabilization" of membranes.

Protective effect of cinnarizine against convulsive elevation of nonesterified fatty acids in CNS.

In the brain tissue there exists a highly active "pool" of nonesterified fatty acids (NEFA). The greatest part of NEFA comes from the fraction of nerve endings. It was evidenced that the model convulsive activity provoked by Bemegrid increased NEFA in the brain cortex, hypothalamus and brain stem.

The effect of delta-9-tetrahydrocannabinol (delta-9-THC) on the release of nonesterified fatty acids in various brain structures.

There was studied the effect of repeated administration of delta-9-THC (10 mg/kg per os for two days) on the level of nonesterified fatty acids (NEFA) in the brain structures of laboratory rats. Palmitic acid, stearic acid, oleic acid and arachidonic acid were estimated with the help of gas chromatography after preliminary separation by TLC. After administration of delta-9-THC, the levels of NEFA decreased in the brain cortex, the brain stem and most in the hypothalamus.

Perspectives of physostigmine as antidote by toluene intoxication.

The administration of physostigmine (0.3 mg/kg s.c.

Mechanism of psychotropic effect of toluene.

An acute 30 min exposure to toluene vapours (0.32 mmol/l of the air) provoked in the rat brain a decrease of total nonesterified fatty acids (arachidonic acid -21.7%, oleic acid -18.

The effect of phospholipase A2 and cyclooxygenase inhibition on free fatty acids in the brain.

The levels of NEFA (oleic acid, palmitic acid, stearic acid, arachidonic acid) were studied in the brain cortex, hypothalamus and the brain stem of rat brain. Inhibition of phospholipase A2 by Delagil (Mepacrinium dichloratum CsL 3) decreased statistically significantly the values of arachidonic aci (-5.5%) in the brain cortex and the values of stearic acid (-11.

The possibilities of clinical application of physostigmine.

A survey of experimental studies dealing with physostigmine in the course of seventeen years is completed by authors' own experience with physostigmine application in practice.