Exploring the Potent Roles of an Internally Translated Truncated Connexin-43 Isoform.

Connexins are a family of transmembrane proteins that form membrane channels [...

Microbial inheritance through seed: a clouded area needs to be enlightened.

Seed endophytes are actively used by the mother plant as both reservoir and vector of beneficial microbes. During seed dormancy endophytes experience significant physiochemical changes and only competent endophytes could colonise successfully in seeds and some of them act as obligate endophyte that are transmitted vertically across generations. The adaptive nature of endophytes allows them to switch lifestyles depending on environment and host conditions.

The geographic scale of population-level variation in growth and nodulation differs for two species of prairie clover.

Premise: Prairies are among the most threatened biomes due to changing patterns of climate and land use, yet information on genetic variation in key species that would inform conservation is often limited. We assessed evidence for the geographic scale of population-level variation in growth of two species of prairie clover and of their symbiotic associations with nitrogen-fixing bacteria.

Methods: Seed representing two species, Dalea candida and D.

A truncated isoform of Connexin43 caps actin to organize forward delivery of full-length Connexin43.

While membrane proteins such as ion channels continuously turn over and require replacement, the mechanisms of specificity of efficient channel delivery to appropriate membrane subdomains remain poorly understood. GJA1-20k is a truncated Connexin43 (Cx43) isoform arising from translation initiating at an internal start codon within the same parent GJA1 mRNA and is requisite for full-length Cx43 trafficking to cell borders. GJA1-20k does not have a full transmembrane domain, and it is not known how GJA1-20k enables forward delivery of Cx43 hemichannels.

The power of friends in reducing sexual assault risk in college women: A preliminary test of dyad-based motivational intervention approach.

Objective: Sexual assault (SA) is unfortunately common in U.S. college campuses.

Effects of a Digital Intervention to Improve DASH and Blood Pressure Among US Adults.

Background: Dietary Approaches to Stop Hypertension (DASH) is a recommended first-line treatment for adults with hypertension, yet adherence to DASH is low.

Purpose: To evaluate the efficacy of a digital health intervention (DHI), compared with attention control, on changes in DASH adherence and blood pressure among adults with hypertension.

Methods: Nourish was a 12-month, parallel, 2-arm, randomized controlled trial of a virtually delivered DHI.

Review: Recent advances in unraveling the genetic architecture of kernel row number in maize.

Kernel row number (KRN) is an important trait in maize that significantly impacts maize yield. The high heritability of KRN underscores its significance in maize breeding programs. In this review, we summarize recent advances in understanding the mechanisms underlying the formation, differentiation, and regulation of KRN in maize.

Interventions for improving treatment adherence in young people with inflammatory bowel disease (IBD): A systematic review of behaviour change theory and behaviour change techniques.

Treatment adherence is important but challenging for young people with inflammatory bowel disease (IBD). Behavioural interventions may support adherence, leading to improved condition management. This review aimed to evaluate interventions designed to improve treatment adherence in young people (aged 13-18) with IBD and identify their use of behaviour change theory and behaviour change techniques (BCTs).

Coming to terms with climate change: a glossary for climate change impacts on mental health and well-being.

Climate change is a major threat to global health. Its effects on physical health are increasingly recognised, but mental health impacts have received less attention. The mental health effects of climate change can be direct (resulting from personal exposure to acute and chronic climatic changes), indirect (via the impact on various socioeconomic, political and environmental determinants of mental health) and overarching (via knowledge, education and awareness of climate change).

Combined Genome-Wide Association Study and Linkage Analysis for Mining Candidate Genes for the Kernel Row Number in Maize ( L.).

Kernel row number (KRN) is one of the key traits that significantly affect maize yield and productivity. Therefore, investigating the candidate genes and their functions in regulating KRN provides a theoretical basis and practical direction for genetic improvement in maize breeding, which is vital for increasing maize yield and understanding domestication. In this study, three recombinant inbred line (RIL) populations were developed using the parental lines AN20, YML1218, CM395, and Ye107, resulting in a multiparent population comprising a total of 490 F9 RILs.

Nucleo-cytoplasmic environment modulates spatiotemporal p53 phase separation.

Liquid-liquid phase separation of various transcription factors into biomolecular condensates plays an essential role in gene regulation. Here, using cellular models and in vitro studies, we show the spatiotemporal formation and material properties of p53 condensates that might dictate its function. In particular, p53 forms liquid-like condensates in the nucleus of cells, which can bind to DNA and perform transcriptional activity.

Cardiac bridging integrator 1 gene therapy rescues chronic non-ischemic heart failure in minipigs.

Heart failure (HF) is a major cause of mortality and morbidity worldwide, yet with limited therapeutic options. Cardiac bridging integrator 1 (cBIN1), a cardiomyocyte transverse-tubule (t-tubule) scaffolding protein which organizes the calcium handling machinery, is transcriptionally reduced in HF and can be recovered for functional rescue in mice. Here we report that in human patients with HF with reduced ejection fraction (HFrEF), left ventricular cBIN1 levels linearly correlate with organ-level ventricular remodeling such as diastolic diameter.

ALS molecular subtypes are a combination of cellular, genetic, and pathological features learned by deep multiomics classifiers.

Amyotrophic Lateral Sclerosis (ALS) is a complex syndrome with multiple genetic causes and wide variation in disease presentation. Despite this general heterogeneity, several common factors have been identified. For example, nearly all patients show pathological accumulations of phosphorylated TDP-43 protein in affected regions of the motor cortex and spinal cord.

COVID-19 risk by work-related factors: pooled analysis of individual linked data from 14 cohorts.

Background: SARS-CoV-2 infection rates vary by occupation, but the association with work-related characteristics (such as home working, keyworker or furlough) are not fully understood and may depend on ascertainment approach. We assessed infection risks across work-related characteristics and compared findings using different ascertainment approaches.

Methods: Participants of 14 UK-based longitudinal cohort studies completed surveys before and during the COVID-19 pandemic about their health, work and behaviour.

Structure-based pharmacophore modelling for ErbB4-kinase inhibition: a systematic computational approach for small molecule drug discovery for breast cancer.

ErbB2 kinase is a key target in approximately 20% of breast cancer cases; however, ErbB2-positive cells may shift their dependence to ErbB4 upon developing resistance to ErbB2 inhibitors. Targeting ErbB4 presents a viable strategy to address this challenge. This study employs a comprehensive approach combining structure-based pharmacophore modelling, molecular docking, and MM-GBSA calculations to identify novel ErbB4 kinase inhibitors.

Self-Protection in the Social Context: A Daily-Level Examination of Young Adult Women's Perceived Need for and Engagement in Sexual Assault Protective Behavioral Strategies.

Objective: Young adult women naturalistically engage in sexual assault protective behavioral strategies (SA-PBS) in an effort to reduce their risk of sexual assault. Despite well-documented sociocontextual risks for sexual assault, it remains largely unknown whether and how social contexts influence SA-PBS use. The present study characterized relations of women's alcohol use and interpersonal contexts with SA-PBS engagement across social events, framed according to Routine Activity Theory.

Discovery of candidate genes enhancing kernel protein content in tropical maize introgression lines.

Background: In this study, tropical maize germplasms were used to construct a multiparent population (MPP) to identify novel genes associated with kernel protein content. The kernel protein content was quantified using near-infrared spectroscopy (NIRS) in the MPP, which was evaluated in three different environments.

Results: QTL mapping revealed 23 significant QTLs tightly linked to kernel protein content, with phenotypic variation ranging from 8.

Hepatocellular senescence induces multi-organ senescence and dysfunction via TGFβ.

Cellular senescence is not only associated with ageing but also impacts physiological and pathological processes, such as embryonic development and wound healing. Factors secreted by senescent cells affect their microenvironment and can induce spreading of senescence locally. Acute severe liver disease is associated with hepatocyte senescence and frequently progresses to multi-organ failure.

QTL mapping and genome-wide association analysis reveal genetic loci and candidate gene for resistance to gray leaf spot in tropical and subtropical maize germplasm.

Using QTL mapping and GWAS, two candidate genes (Zm00001d051039 and Zm00001d051147) were consistently identified across the three different environments and BLUP values. GWAS analysis identified the candidate gene, Zm00001d044845. These genes were subsequently validated to exhibit a significant association with maize gray leaf spot (GLS) resistance.

Fine mapping of a major co-localized QTL associated with self-incompatibility identified in two F populations (broccoli × cauliflower and cauliflower × Chinese kale).

A major QTL responsible for self-incompatibility was stably identified in two F populations. Through fine mapping and qRT-PCR analysis, ARK3 emerged as the most promising candidate gene, playing a pivotal role in regulating self-incompatibility in Brassica oleracea. Self-incompatibility (SI) is a common phenomenon in Brassica oleracea species, which can maintain genetic diversity but will also limit seed production.

Recurrent Syncope as a Rare Initial Manifestation of Diffuse Large B-Cell Lymphoma in the Cervical Region.

BACKGROUND Diffuse large B-cell lymphoma is the most prevalent form of non-Hodgkin lymphoma, representing around a quarter of newly diagnosed cases of B-cell non-Hodgkin lymphoma. Diffuse large B-cell lymphoma is a disease that affects mostly older persons, with a median presentation in the 7th decade of life. Clinically, it has a variety of presentations, ranging from constitutional symptoms to local or systemic pressure effects caused by a rapidly growing mass.

Socioeconomic Deprivation Correlates With Incomplete Radiotherapy Treatment in Head and Neck Cancer.

Unlabelled: There is a high burden of socioeconomic deprivation across Merseyside and, along with this, poorer cancer outcomes. The incidence of head and neck squamous cell carcinoma (HNSCC) within this region is higher than the national average and there are often additional complexities to individual treatment pathways such as poor health literacy, lack of social support and transport options which can impact on adherence to prescribed treatments.

Aims: This work aims to understand the impact of deprivation on patients diagnosed with HNSCC undergoing chemoradiotherapy (CRT) or radiotherapy (RT) treatment by identifying barriers to adherence.

Development of the First Patient-Reported Experience Measure (PREM) for Hearing Loss in Audiology Care-My Hearing PREM.

Context: Patient-reported experience measures (PREMs) provide important insights into the challenges experienced when living with a chronic condition. Although patient-reported outcome measures (PROMs) exist in audiology, there are no validated PREMs to help clinicians understand patient perspectives and identify areas where patients may need additional support or interventions.

Objective: The aim of this study was to develop and evaluate content for the new 'My Hearing PREM', which captures lived experiences of hearing loss from patients' perspectives.

Modeling compound lipid homeostasis using stable isotope tracing.

Lipids represent the most diverse pool of metabolites found in cells, facilitating compartmentation, signaling, and other functions. Dysregulation of lipid metabolism is linked to disease states such as cancer and neurodegeneration. However, limited tools are available for quantifying metabolic fluxes across the lipidome.