Binding of 4-methylumbelliferyl beta-D-galactosyl-(1 leads to 3)-N-acetyl-beta-D-galactosaminide to peanut agglutinin. Characterisation and application in substitution titrations.

Binding of 4-methylumbelliferyl-2-acetamido-2-deoxy-3-O-(beta-D-galactopyranosyl) beta-D-galactopyranoside [MeUmb beta Gal(beta 1 leads to 3)GalNAc] to peanut agglutinin was characterized by equilibrium dialysis and by measurement of the increase in ultraviolet absorption or fluorescence of the chromophoric glycoside upon continuous titration with excess of the lectin. All data in the 4-30 degrees C range correspond to delta G = -(26.5 +/- 0.

Representation of social work agencies: new definition, special issues, and practice model.

Social Work administrators currently must respond to very diverse audiences in representing their agencies and programs. While skills in public relations, reporting, and community relations are viewed as essential, professional social work literature provides only limited conceptualizations and practice models. The authors propose a new definition of representation in social work agencies and suggest functional areas within which organized activities can be developed.

Sugar-specific endocytosis of glycoproteins by Lewis lung carcinoma cells.

Lewis lung carcinoma cells from tumors, metastasis nodules, or from culture bind fluorescent derivatives of neoglycoproteins containing alpha-D-glucose residues: This binding is competitively inhibited by neoglycoproteins containing alpha-D-glucose, by mannan, and by several other neoglycoproteins. Cell binding and uptake of the fluorescent derivatives of the neoglycoproteins was quantified by lysing the cells with an alkylpolyol (MAC 19 or MAC 18) and measuring the fluorescence intensity of the supernatant. The amount of cell-associated neoglycoprotein was higher at 37 degrees C than at 4 degrees C with LLC from tumor.

Long-term effectiveness of the immunotherapy of anal condyloma acuminatum.

In a seven-year period, 200 patients with persistent or recurrent anal and perianal condyloma acuminatum were treated by immunotherapy. There were 120 primary and 80 recurrent cases in the study. Autogenous vaccine was prepared for each patient and injected subcutaneously, 0.

Acquisition of mitogenic responsiveness by nonresponding lymphocytes upon insertion of appropriate membrane components.

The effect of insertion of plasma membrane components from lymphocytes responding to mitogens into the membranes of nonresponding cells using Sendai virus envelopes as vehicles was examined. T cells modified by B membranes were stimulated by lipopolysaccharide (LPS) to proliferate as well as to produce interleukin-2 activity. B cells modified by T membranes were stimulated by concanavalin A to proliferate and to produce interleukin-2 activity.

Mannose-specific adherence of Escherichia coli freshly excreted in the urine of patients with urinary tract infections, and of isolates subcultured from the infected urine.

The mannose-specific adherence to yeast cells of Escherichia coli excreted in the urine of patients with urinary tract infections was compared with that of isolates from the same urine after growth of the bacteria in broth. The results revealed that although E. coli excreted in only 2 of 24 urine specimens exhibited mannose-specific adherence, about half of the broth cultures from these specimens did so.

Dissociation and reassembly of Escherichia coli type 1 pili.

Escherichia coli type 1 pili, which mediate the mannose-sensitive adherence of the bacterium to eucaryotic cells, are comprised of very stable arrays of pilin protein subunits (molecular weight, approximately 17,000). Previous methods for the dissociation of pili caused their irreversible denaturation. We have found that incubation of pili in saturated guanidine hydrochloride at 37 degrees C led to their complete dissociation, as evidenced by nephelometry and electron microscopy.

Primary structure of the carbohydrate chain of soybean agglutinin. A reinvestigation by high resolution 1H NMR spectroscopy.

In an earlier report (Lis, H., and Sharon, N. (1978) J.

Human B lymphocytes form rosettes after insertion of T lymphocyte membrane constituents.

Isolated plasma membranes from human thymocytes or peripheral T lymphocytes upon their reconstitution with envelope glycoproteins of Sendai virus, afforded membrane vesicles that fused efficiently with human peripheral B cells. The b cells thus obtained acquired the ability to form regular rosettes at 4 degrees C with sheep red blood cells. However, only B cells modified by fusion with thymocyte membranes formed stable rosettes at 37 degrees C, a property which distinguished thymocytes from peripheral T cells.

Lipid-mediated hemagglutination and its relevance to lectin-mediated agglutination.

Oleic acid and dioleoyl phosphatidic acid at low concentrations (20 and 0.5 mu g/ml, respectively) agglutinate rabbit and rat erythrocytes, while dioleoyl phosphatidylcholine is not hemagglutinating up to 0.5 mg/ml.

Bacterial adherence to cell surface sugars.

Bacterial adherence to animal cell surfaces is of interest because of its relation to pathogenicity and the insight it provides into determinants of intercellular recognition. The attachment of various strains of Escherichia coli and Salmonella spp. to epithelial cells and phagocytes is inhibited by D-mannose, and the adherence of other bacteria is inhibited by sugars such as L-fucose and D-galactose, suggesting that sugar-mediated adherence is widespread.

Alteration of lymphocyte surface properties by insertion of foreign functional components of plasma membrane.

We report the successful coreconstitution of solubilized lymphocyte plasma membranes and Sendai virus envelopes into membrane vesicles that possess the ability to fuse efficiently with mouse splenocytes of thymocytes to give fully viable cells with modified surface properties. Integration of donor membrane components into the acceptor cells was demonstrated by chemical, morphological, and immunological methods. Over 40% of the label from vesicles coreconstituted with radiolabeled (with 125I or 3H) lymphocyte membranes was incorporated into the fused cells.