Mitochondrial Protein Density, Biomass, and Bioenergetics as Predictors for the Efficacy of Glioma Treatments.

The metabolism of glioma cells exhibits significant heterogeneity and is partially responsible for treatment outcomes. Given this variability, we hypothesized that the effectiveness of treatments targeting various metabolic pathways depends on the bioenergetic profiles and mitochondrial status of glioma cells. To this end, we analyzed mitochondrial biomass, mitochondrial protein density, oxidative phosphorylation (OXPHOS), and glycolysis in a panel of eight glioma cell lines.

Rituximab and reduced-intensity chemotherapy in children and adolescents with mature B-cell lymphoma: interim results for 231 patients enrolled in the second Russian-Belorussian multicentre study B-NHL-2010M.

The value of adding rituximab to chemotherapy in children with aggressive B-cell non-Hodgkin lymphoma (B-NHL) is still insufficiently studied. We enrolled 231 patients [mean age 9 years old (range 2-17); male:female ratio 3·4:1] with Burkitt (BL, 179 patients, 76·7%), diffuse large B-cell (32 patients, 14%), primary mediastinal B-cell (14 patients, 6%), and other (6 patients, 2·6%) B-cell lymphomas in a prospective study of immuno-chemotherapy. Stages were I-II in 32% and III-IV in 68% of the patients.

[Age-related characteristics of the efficacy of different glucocorticosteroids in the therapy of acute lymphoblastic leukemia].

Aim: To determine predictors for decision-making on a differential approach to choosing glucocorticosteroids (GCS) for children and adolescents with acute lymphoblastic leukemia (ALL).

Subjects And Methods: The analysis covered 1064 primary patients aged to 1 to 18 years with ALL who had been registered at the clinics of Russia and Belorussia in April 2002 to November 2006. Before induction therapy, the patients were randomized into a dexamethasone (DEXA) 6 mg/m2 group (n=539) and a methylprednisolone (MePRED) 60 mg/m2 one (n=525).

[Effects of local induction of ipt-gene in roots on cytokinins content in leaf cells tobacco plants].

Identification of cytokinins in differentiated leaf cells has received little attention. We have carried out immunohistochemical localization of cytokinins in leaves of transgenic tobacco plants in which the level of increased due to induced in their roots the expression of ipt-gene controlling cytokinin synthesis. Immuno-labeling of cytokinins with the help of antibodies raised against zeatin riboside was characteristic of mesophyll cells.

[Characteristics of the lipid transport system in psoriasis].

Cholesterol of high density lipoprotein (HDL) subfractions (HDL2 and HDL3), activity of cholesterol ester transport protein (CETP), and standard lipoprotein parameters (cholesterol, triglycerides, HDL cholesterol) were measured in plasma of 192 patients with various psoriasis forms, which included simple "distributed psoriasis", erythrodermic and arthropathic psoriasis. Among psoriatic patients unusually high percent of persons with both hypo- and hyper-cholesterolemia was observed. The same situation was observed with frequency of cases with both low and high plasma HDL cholesterol levels, the distribution being depending from disease severity.

[Plasmosorption in combined therapy of psoriasis].

Combined therapy with plasmosorption has been performed in 22 patients aged 18-65 years suffering from different variants of psoriasis diagnosed 5 to 24 years before. Stable remission was achieved in 16 patients who exhibited normalization of peripheral blood characteristics including total protein and protein plasma fractions, lowering of CIC and middle molecular peptides concentration. The response was also obtained in severe psoriasis (psoriatic erythrodermia and arthropathic psoriasis).

[Features of distribution of free and esterified cholesterol in the epidermis, biological membranes and plasma lipoproteins in psoriasis].

The parameters of cholesterol (CS) metabolism in the epidermis, blood plasma lipoproteins, and red cell membranes were assessed in patients with psoriasis. The levels of free and total CS in extracts from the surface of epidermis, in plasma lipoproteins, and in plasma triglycerides were measured, and the molar ratio CS/ phospholipids (CS/PL index) in red cell membranes assessed in 194 patients with psoriasis of different severity. The levels of free and total CS in the epidermis were found to increase as the psoriatic process grew in severity.

[Free and esterified epidermal cholesterol in psoriasis].

The levels of epidermal total, free and esterified cholesterol were studied in psoriatic patients. Lipids were isolated from the areas of psoriatic lesions and from the normally appearing epidermis by the authors' non-invasive surface extractive method. The quantity of cholesterol was given in microgram per square centimeter.

[A method of determination of cholesterol contents on the surface of the human skin and prospects of its use. II. Psoriasis].

A noninvasive method for cholesterol extraction from the surface layers of the epidermis is suggested. Levels of cholesterol and its fractions were measured in extracts obtained from the foci of psoriasis and apparently intact skin of 105 patients with psoriasis. Cholesterol levels were found noticeably increased, mainly at the expense of its free fraction in involved foci and in apparently intact skin.

[The treatment of psoriasis by efferent methods on an outpatient basis].

In addition to conventional outpatient treatment 165 psoriasis patients underwent hemosorption (100 cases) or plasmapheresis (65 cases). The efferent methods used proved beneficial in 99 (60%) of the patients that got to have normal hemograms, reduced CIC and middle molecular weight peptides levels in blood. The response depended on the disease duration irrespectively of the method of efferent treatment.

[Enterosorption in the combined therapy of psoriasis patients].

The authors consider the clinical efficacy of enterosorption, carried out in 112 patients with various forms of psoriasis. Indications for and contraindications to this treatment modality are defined. Changes in the level of endogenous intoxication in the paramecium test over the course of enterosorption treatment are analyzed.