Quantitation of electrophoretic eluted proteins.

Quantitation of stained, electroeluted proteins by the classical Lowry and Bradford protein assay is not possible because of some different interferences. In particular we have found that the substance interfering in the Lowry method cannot be removed by trichloroacetic acid precipitation nor can be compensated for by the appropriate blank. Interferences in the Bradford protein assay are due to detergents and pH of the protein buffer as well as to Coomassie brilliant blue R250 electroeluted with the protein sample.

Purification, properties, and evidence for two subtypes of human placenta hexokinase type I.

In human placenta 85% of total hexokinase activity (EC 2.7.1.

Decay mechanisms of rabbit hexokinase during reticulocyte maturation.

In rabbit erythrocytes hexokinase (EC 2.7.1.

Role of hexokinase in the regulation of glucose metabolism in human erythrocytes.

Red blood cell glucose metabolism was studied in erythrocytes from a patient with trisomy 10 p which resulted in + 50% hexokinase specific activity, in normal controls and in cases of heterozygous hexokinase deficiency. The results obtained show that the hexokinase activity level is an important factor in the control of the erythrocyte's glycolytic rate while having no appreciable effect on the hexose monophosphate pathway under resting conditions. No clear conclusion could be drawn when an oxidative stress was present.

Rabbit red blood cell hexokinase. Decay mechanism during reticulocyte maturation.

In rabbit reticulocytes, the hexokinase (EC 2.7.1.

Human erythrocyte hexokinases are immunologically related.

Human erythrocytes contain three major hexokinase isoenzymes eluted by DE-52 chromatography between hexokinase type I and type II. Cross-reactivities of these isoenzymes were studied by means of a monospecific rabbit antibody against purified human placenta hexokinase type I. It was shown that the three hexokinase isoenzymes were immunologically related, supporting the concept of a postsynthetic mechanism(s) as their origin.

Rat red blood cell hexokinase purification, properties and age-dependence.

Rat erythrocytes, in contrast to red blood cells from other mammals, have been shown to contain only one hexokinase isozymic form identified as type I by chromatographic and kinetic properties. Rat reticulocytes contain 3.6-times the hexokinase activity found in mature erythrocytes but exactly the same isozyme.

Location of central venous catheters in children by endocavitary ECG: A new technique.

A new technique to establish the position of a central venous catheter (CVC) by endocavitary ECGraphy is described. The principle is based on the fact that the catheter itself may be considered as an electrical conductor by means of its liquid content (saline solution), therefore acting as an exploring electrode. The intrinsic deflection of the P wave shows the proper position of the CVC tip at the sinus node level.

[Echographic study of the embryonal and fetal hand].

The morphological aspects of the limbs as well as several details in the forearm and in the hand were observed from the seventh week of gestation on by routine sonographic examination of the embryo and the fetus. This examination was performed once a week beginning in the first months of life. The authors emphasize the value of sonography in the detection of fetal and embryonic malformations of the upper limb.

Purification and properties of the cytoplasmic hexokinase from rabbit brain.

About 90% of the total hexokinase activity in rabbit brain was found to be associated with mitochondria while the remaining part was found in the cytosolic fraction. The soluble enzyme was purified 4,700-fold to near homogeneity by a combination of ion-exchange chromatography, dye-ligand chromatography and affinity chromatography. The purified enzyme showed a specific activity of 110 units/mg of protein and was obtained in 70% yield.

[Purification and properties of cytoplasmic and mitochondrial hexokinase from the rabbit brain].

The soluble Hexokinase from rabbit brain was purified 4,700-fold to near homogeneity by a combination of ion-exchange chromatography, dye-ligand chromatography and affinity chromatography. The purified enzyme showed a specific activity of 110 units/mg of protein and was obtained in 70% yield. The properties of the purified cytoplasmic hexokinase were compared with those of the solubilized mitochondrial enzyme.

[Campylobacter fetus subsp. jejuni: etiological, epidemiological and preventive aspects].

The Authors, after a brief introduction, examine on the basis of the most recent literature, the taxonomy and the microbiological features of Campylobacter fetus subsp. jejuni, giving a detailed description of the chemical composition, of the biochemical, physiological and nutritional characters, of the antigenic composition and of the cultural methods. Later on, they state the problems arising from the epidemiology of the infections caused by this germ either in man or in animals, examining the most relevant sources of infection.

Effects of buffers and pH on rabbit red blood cell hexokinase.

The kinetic properties of rabbit red blood cell hexokinase in different buffer systems have been studied. At pH 8.0 the reaction velocity (v) is about 30% higher in glycylglycine compared to Tris, Tea, Hepes or ammonium acetate buffers.

[Epidemiology of multiresistant S. aureus in a burn center].

Multi-resistant S. aureus, have been isolated from wounds of patients in the burn unit of the University of Parma. The strains were resistant to penicillin (85%), methicillin (30%), gentamycin (63%), rifampicin (41%), cotrimoxazole (38%), lincomycin (39%), tetracycline (73%), erythromycin (66%) and cephaloridine (11%).

[Ketamine hydrochloride by continuous drip intravenous infusion in thoracic surgery].

The Authors report an open trial on 50 patients anaesthetized with Ketamine hydrochloride, by continuous drip infusion progressively slowed, with the aid of Fentanil, in thoracic surgery. The proposed anaesthetic technique proved to be excellent as for as analgesia during and after operation as well cardiovascular stability and lackness of side effects is concerned. Recovery from anaesthesia was quick and extremely quite and free from side effects which are common in Ketamine anaesthesia.