Tuning the lanthanide binding tags for preferential actinide chelation: an all atom molecular dynamics study.

The present study focuses on designing mutant peptides derived from the lanthanide binding tag (LBT) to enhance selectivity for trivalent actinide (An) ions over lanthanide (Ln) metal ions (M). The LBT is a short peptide consisting of only 17 amino acids, and is known for its high affinity towards Ln. LBT was modified by substituting hard-donor ligands like asparagine (ASN or N) and aspartic acid (ASP or D) with softer ligand cysteine (CYS or C) to create four mutant peptides: M-LBT (wild-type), M-N103C, M-D105C, and M-N103C-D105C.

Counting efficiencies of shadow shield whole body counter for adult and paediatric reference computational phantoms.

Shadow Shield Whole Body Counter (SSWBC) is used to estimate internal dose of radiation workers due to the intake of fission and activation products. The SSWBC geometry was numerically modelled in FLUKA code. The computational model was validated by comparing the experimental and simulated counting efficiencies (CEs), also known as response, using Bhabha Atomic Research Centre (BARC) reference BOttle Mannequin Absorption (BOMAB) phantom.

Ambient ultraviolet-B exposure brings quantum changes in phenotypic and molecular signatures of the embryo of a high-altitude fish, Tor putitora.

Effects of environmentally relevant ultraviolet B (UVB) light on the embryonic development of a high-altitude dwelling endangered fish, Tor putitora (golden mahseer), were investigated for the first time. For that, three sets of embryos (in triplicates) were exposed to various ambient UVB light doses (1, 2 and 3 W/m, corresponding to 3.6, 7.

Investigation on the impact of the spatial arrangement of individuals in a standing-type in-vivo monitoring system.

An automated standing type Quick Scan Whole Body Monitor (QS-WBM) has been developed for the measurement of internal radioactive contamination due to high energy photon (HEP) emitters (E >200 keV). Individuals are monitored while standing on a platform inside QS-WBM at specified reference position. Instances may occur where individuals deviate from their monitoring position, potentially leading to errors in the measurement of the body content.

Estimation and analysis ofvalues forI using paediatric mesh type reference computational phantoms.

This study examines the effect of paediatric mesh-type reference computational phantoms on organvalues resulting from radioiodine (I) intake. Using Geant4, we estimatedIvalues for 30 radiosensitive target tissues due to emission from the thyroid (Target ← Thyroid) in these phantoms. Our results show thatvalues differ between male and female phantoms of the same age andvalues also decrease as phantom age increases.

Modelling of constant and realistic chronic intake scenarios and their implications.

Chronic inhalation intake of radionuclides is possible for radiation workers in the nuclear industry. The International Commission on Radiological Protection (ICRP) provides organ retention fractions as well as daily urinary and fecal excretion for chronic intake of various radionuclides. In this study, organ retention fractions and daily urinary excretion for chronic intake were estimated for constant chronic intake (CCI) and realistic chronic intake (RCI) scenarios.

Estimating Uncertainty in Actinide Intake Due to Unknown Time of Exposure.

Bioassay of radiation workers handling actinides in powder form is carried out annually under routine monitoring program. In case of routine monitoring, as the day of intake is unknown, it is assumed that intake has occurred at the midpoint of monitoring interval. However, an uncertainty is introduced in intake estimation due to this assumption.

Sociocultural aspects of delays in diagnosis among tuberculosis-diabetes comorbid patients in Satara, India: Its implications for the implementation of the national framework for joint tuberculosis-diabetes collaborative activities.

Background: Tuberculosis(TB) and Diabetes comorbidity is an emerging public health problem in India. Delays in diagnosing TB or Diabetes would lead to adverse outcomes among comorbid patients, and attempts must be made to reduce these delays. Against this background, the study has been undertaken to clarify the role of sociocultural factors in determining diagnostic delays for TB and Diabetes among comorbid patients.

Submandibular and sublingual salivary gland involvement in adenoid cystic carcinoma.

Adenoid cystic carcinoma (AdCC) is a malignant salivary gland neoplasm that presents as an indolent but aggressive neoplasm. AdCC histogenesis is linked to the intercalated ducts of the salivary glands, equally affecting the major and minor glands. AdCC is associated with distant metastasis, most commonly to the lungs, and a high recurrence rate.

Speciation-specific chromium bioaccumulation and detoxification in fish using hydrogel microencapsulated biogenic nanosilver and zeolite synergizing with biomarkers.

Grass carp intestinal waste-mediated biosynthesized nanosilver (AgNPs) was valorized using guaran and zeolite matrices, resulting in AgNPs-guaran, AgNPs-zeolite, and AgNPs-guaran -zeolite composites. The valorized products were examined using Environmental Scanning Electron Microscopy, Energy Dispersive X-ray analysis and X-ray Diffraction analysis to confirm uniform dispersion and entrapment of AgNPs within the matrixes. These valorized products were evaluated for their efficacy in detoxifying the ubiquitous and toxic hexavalent chromium (Cr) in aquatic environments, with Anabas testudineus exposed to 2 mg l of Cr for 60 days.

Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis.

Interhomolog recombination in meiosis is mediated by the Dmc1 recombinase. The Mei5-Sae3 complex of Saccharomyces cerevisiae promotes Dmc1 assembly and functions with Dmc1 for homology-mediated repair of meiotic DNA double-strand breaks. How Mei5-Sae3 facilitates Dmc1 assembly remains poorly understood.

Predicting over-the-counter antibiotic use in rural Pune, India, using machine learning methods.

Objectives: Over-the-counter (OTC) antibiotic use can cause antibiotic resistance, threatening global public health gains. To counter OTC use, this study used machine learning (ML) methods to identify predictors of OTC antibiotic use in rural Pune, India.

Methods: The features of OTC antibiotic use were selected using stepwise logistic, lasso, random forest, XGBoost, and Boruta algorithms.

LncRNA-mediated orchestrations of alternative splicing in the landscape of breast cancer.

Alternative splicing (AS) is a fundamental post-transcriptional process in eukaryotes, enabling a single gene to generate diverse mRNA transcripts, thereby enhancing protein variability. This process involves the excision of introns and the joining of exons in pre-mRNA(s) to form mature mRNA. The resulting mature mRNAs exhibit various combinations of exons, contributing to functional diversity.

Metatranscriptomic assessment of diarrhoeic faeces reveals diverse RNA viruses in rotavirus group A infected piglets and calves from India.

RNA viruses are a major group contributing to emerging infectious diseases and neonatal diarrhoea, causing morbidity and mortality in humans and animals. Hence, the present study investigated the metatranscriptomic-derived faecal RNA virome in rotavirus group A (RVA)-infected diarrheic piglets and calves from India. The viral genomes retrieved belonged to in both species, while were found only in piglets.

I dose coefficients for a reference population using age-specific models.

Age-specific dose coefficients are required to assess internal exposure to the general public. This study utilizes reference age-specific biokinetic models of iodine to estimate the total number of nuclear disintegrations ã(,τ) occurring in source regions () during the commitment time (τ). Age-specific S values are estimated for 35 target regions due toI present in 22using data from 10 paediatric reference computational phantoms (representing five ages for both sexes) published recently by the International Commission of Radiation Protection (ICRP).

When a synonymous mutation breaks the silence in a thalassaemia patient.

Synonymous mutations were considered to lack functional roles in human diseases; however, distinguishing deleterious synonymous mutations from benign ones is still a challenge. In this article, we identified a deleterious synonymous mutation β-codon 16 (C>T). HBB: c.

Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India.

Astroviruses (AstV) and adenoviruses (AdV) are associated with diarrhoea in young animals. However, the epidemiology and genetic diversity of AstVs and AdVs in animals is not well studied. Hence, the present study was conducted to detect and characterize AstVs and AdVs in calves, piglets and puppies from Western Maharashtra, India.

Small size silver nanoparticles loaded with glycoside rich portion of Boerhavia Diffusa Linn. promotes wound healing: in-silico and in-vivo studies.

Silver nanoparticles (Ag-NPs) are increasingly used in various fields, including medicine, owing to their unique physicochemical properties. Due to their smaller size, the contact with biological components is increased, and consequently, it performs better as an antibacterial and antimicrobial. In this study, the authors have focused on the synthesis of small-sized spherical silver nanoparticles (Ag-NPs) by a chemical reduction method using two different capping agents and concentrations of AgNO as a precursor.

Application of Rupantaran software to Sri Lankan hospitals: an innovative tool developed to merge population-based cancer registry data into CanReg5.

The purpose of a population-based cancer registry is to provide information on the disease burden for cancer control planning and is essential in studies on assessing the effectiveness of prevention, early detection, screening and cancer care interventions, where implemented. Sri Lanka is one of the Member States of the World Health Organisation's South-East Asia Region and receives technical support for cancer registration from the International Agency for Research on Cancer (IARC), and the IARC Regional Hub based at the Tata Memorial Centre in Mumbai, India. For data management of cancer registry records, Sri Lanka National Cancer Registry (SLNCR) uses the open-source registry software tool, CanReg5, as developed by IARC.

A rationale for the rapid extraction of ultra-low-level uranyl ions in simulated bioassays regulated by Mn-dopants over magnetic nanoparticles.

Although the sorption of uranyl ions and other heavy metal ions over magnetic nanoparticles is well reported, the parameters governing the sorption process over the magnetic nanoparticles have not been clearly enumerated. However, to increase the efficiency of the sorption over the surface of these magnetic nanoparticles, it is essential to understand the different structural parameters that are involved in the sorption process. The sorption of uranyl ions and other competitive ions in simulated urine samples at different pH was effectively accomplished over magnetic nanoparticles of FeO (MNPs) and Mn-doped FeO (Mn-MNPs).

The role of conserved amino acid residues of Sae3 in Mei5-Sae3 complex for Dmc1 assembly in meiotic recombination.

Meiotic recombination between homologous chromosomes is promoted by the collaborative action of two RecA homologs, Rad51 and meiosis-specific Dmc1. The filament assembly of Dmc1 is promoted by meiosis-specific Mei5-Sae3 in budding yeast. Mei5-Sae3 shows sequence similarity to fission yeast Sfr1-Swi5, which stimulates DNA strand exchanges by Rad51 as well as Dmc1.

Mitigating abiotic stresses using natural and modified stilbites synergizing with changes in oxidative stress markers in aquaculture.

In-depth mineralogical understanding and characterization are necessary to explore potential applications of clinoptilolites. In this study, the clinoptilolite collected from quarries and identified as stilbite microscopically and spectroscopically was subjected to physical and chemical treatments for the synthesis of modified stilbites, which were further evaluated for determining their removal efficiencies on ammonical contaminant in a predetermined concentration range from various source of aquaculture water namely fish pond, aquaponics and ornamental under laboratory condition. High-resolution transmission electron microscope results revealed that stilbite was rod-shaped in all forms but physically modified stilbite contains some nano-zeolite particles, synthesized probably due to heat treatment.

Image-guidance triggered adaptive radiation therapy in head and neck squamous cell carcinoma: single-institution experience and implications for clinical practice.

Purpose: To report frequency and timing of adaptive radiotherapy (ART) and assess patient, disease, and treatment-related characteristics potentially triggering the need for such adaptive replanning in head and neck squamous cell carcinoma (HNSCC).

Methods: Medical records of HNSCC patients treated with definitive intensity modulated radiation therapy (IMRT) with or without concurrent systemic chemotherapy were reviewed retrospectively to identify patients undergoing image-guidance triggered adaptive replanning. Clinico-demographic characteristics of patients undergoing ART were compared with patients treated without adaptation using the chi-square test.