3D-printed sodium alginate/carbon nanotube/graphene porous scaffolds crosslinked with Ca for high-performance electromagnetic shielding and Joule heating.

High-performance green functional materials have garnered significant interest for electromagnetic interference (EMI) shielding applications, but creating customized, low-density, high-strength and high-efficiency biomass-based shielding materials remains challenging. In this study, lightweight Ca doped sodium alginate (SA) porous scaffolds with a carbon nanotube (CNT)/graphene (Gr) hybrid conductive network were fabricated via direct ink writing (DIW) 3D printing. The SA/CNT/Gr inks with unique rheological properties were formulated and architectures with arbitrarily customized structures could be freely constructed based on the printable inks.

Versatile poly (deep eutectic solvents) electroactive chitosan eutectogel for infected wound healing and monitoring administration.

The treatment and monitoring of infected skin wounds present significant clinical challenges. Herein, a multifunctional poly(deep eutectic solvent) (PDES) electroactive hydrogel is developed by optimizing the components and the ratio of hydrogen donors and acceptors, achieving well wound hemostasis, wound healing, and monitoring administration performace. The PDES hydrogel dressing exhibits mechanical properties, including high toughness, fatigue resistance (over 9000 compression cycles), and superior skin adhesion (over 70 kPa).

Abelacimab versus Rivaroxaban in Patients with Atrial Fibrillation.

Background: Abelacimab is a fully human monoclonal antibody that binds to the inactive form of factor XI and blocks its activation. The safety of abelacimab as compared with a direct oral anticoagulant in patients with atrial fibrillation is unknown.

Methods: Patients with atrial fibrillation and a moderate-to-high risk of stroke were randomly assigned, in a 1:1:1 ratio, to receive subcutaneous injection of abelacimab (150 mg or 90 mg once monthly) administered in a blinded fashion or oral rivaroxaban (20 mg once daily) administered in an open-label fashion.

Prognostic impact of coronary microvascular dysfunction assessed by AMR in acute coronary syndrome patients with chronic kidney disease.

Background: Angiography-derived microcirculatory resistance (AMR) is proposed as a novel, pressure- temperature-wire-free and less-invasive method to evaluate coronary microvascular dysfunction (CMD). This study aims to examine the prognostic role of CMD assessed by AMR in predicting adverse events in acute coronary syndrome (ACS) patients with chronic kidney disease (CKD).

Methods: This retrospective cohort study included ACS with CKD patients in the China-Japan Friendship Hospital from January 2016 to November 2022.

Ultrasensitive ctDNA detection for preoperative disease stratification in early-stage lung adenocarcinoma.

Circulating tumor DNA (ctDNA) detection can predict clinical risk in early-stage tumors. However, clinical applications are constrained by the sensitivity of clinically validated ctDNA detection approaches. NeXT Personal is a whole-genome-based, tumor-informed platform that has been analytically validated for ultrasensitive ctDNA detection at 1-3 ppm of ctDNA with 99.

Hydralazine inhibits cysteamine dioxygenase to treat preeclampsia and senesce glioblastoma.

The vasodilator hydralazine (HYZ) has been used clinically for ~ 70 years and remains on the World Health Organization's List of Essential Medicines as a therapy for preeclampsia. Despite its longstanding use and the concomitant progress toward a general understanding of vasodilation, the target and mechanism of HYZ have remained unknown. We show that HYZ selectively targets 2-aminoethanethiol dioxygenase (ADO) by chelating its metal cofactor and alkylating one of its ligands.

Toward expert-level medical question answering with large language models.

Large language models (LLMs) have shown promise in medical question answering, with Med-PaLM being the first to exceed a 'passing' score in United States Medical Licensing Examination style questions. However, challenges remain in long-form medical question answering and handling real-world workflows. Here, we present Med-PaLM 2, which bridges these gaps with a combination of base LLM improvements, medical domain fine-tuning and new strategies for improving reasoning and grounding through ensemble refinement and chain of retrieval.

The Potency of Cytotoxic Mechanisms of Local Anesthetics in Human Chondrocyte Cells.

Local anesthetics are commonly used in various clinical settings for both prevention and symptom relief. Numerous clinical studies have demonstrated that intra-articular injections of local anesthetics achieve high success rates in orthopedic practices. However, several widely used local anesthetics, including bupivacaine, lidocaine, and ropivacaine, have been shown to exhibit toxicity to chondrocytes, with the underlying mechanisms of chondrotoxicity remaining poorly understood.

Deficiency of Endothelial Impairs Pulmonary Vascular Angiogenesis and Predisposes Pulmonary Hypertension.

Background: Mechanosensitive Piezo1 channel plays a key role in pulmonary hypertension (PH). However, the role of Piezo2 in PH remains unclear.

Methods: Endothelial cell (EC)-specific knockout (, Tek-Cre; ) rats and primarily cultured pulmonary microvascular ECs were used to determine the role of Piezo2 in PH.

Self-healing, deformable and safe integrated supercapacitor enabled by synergistic effect of multiple physical interactions in gel polymer electrolyte with dual-role Co.

With the rapid development of wearable electronic devices, flexible supercapacitors have gained strong interest. However, traditional sandwich supercapacitors have weak interfacial binding, resulting in high interface resistance and poor deformability. Herein, a self-healing integrated supercapacitor based on a polyacrylic acid-polyisodecyl methacrylate-CoSO gel polymer electrolyte (GPE) was developed.

Targeting P4HA1 promotes CD8 T cell progenitor expansion toward immune memory and systemic anti-tumor immunity.

Successful immunotherapy relies on both intratumoral and systemic immunity, which is yet to be achieved for most patients with cancer. Here, we identify P4HA1, encoding prolyl 4-hydroxylase 1, as a crucial regulator of CD8 T cell differentiation strongly upregulated in tumor-draining lymph nodes (TDLNs) and hypoxic tumor microenvironment. P4HA1 accumulates in mitochondria, disrupting the tricarboxylic acid (TCA) cycle through aberrant α-ketoglutarate and succinate metabolism, promoting mitochondria unfitness and exhaustion while suppressing progenitor expansion.

Severe fever with thrombocytopenia syndrome virus was found in Northern Jiangxi Province, China.

Introduction: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease discovered in China in 2009. SFTS monitoring has been carried out since 2010 in mainland China. In recent years, human infection with SFTS virus (SFTSV) has frequently been detected in Jiujiang of Jiangxi Province, Central China.

Adaptive Platform Trials in Stroke.

Clinical trials of treatments for stroke have generally utilized 2-arm, randomized designs to evaluate a single intervention against a control. Running separate clinical trials, with each addressing a single therapeutic question, is resource intensive and slows evidence generation, especially in a field with rapidly expanding treatment options and evolving practices. Platform trials-randomized clinical trials designed to evaluate multiple interventions that may enter and exit the ongoing platform based on a master protocol-accelerate the investigation of multiple therapeutic options within a single infrastructure.

Stretchable and self-healing carboxymethyl cellulose/polyacrylic acid conductive hydrogels for monitoring human motions and electrophysiological signals.

Stretchable conductive hydrogels have attracted great attention in flexible electronics. Nevertheless, conductive hydrogels would suffer from an inevitable damage during use, significantly reducing the reliability and limiting the practicability. Herein, stretchable and self-healing conductive hydrogels are designed form carboxymethyl cellulose (CMC), polyacrylic acid (PAA), and Fe, which are applied for monitoring human motions and electrophysiological signals.

Engineered extrachromosomal oncogene amplifications promote tumorigenesis.

Focal gene amplifications are among the most common cancer-associated mutations but have proven challenging to engineer in primary cells and model organisms. Here we describe a general strategy to engineer large (more than 1 Mbp) focal amplifications mediated by extrachromosomal DNAs (ecDNAs) in a spatiotemporally controlled manner in cells and in mice. By coupling ecDNA formation with expression of selectable markers, we track the dynamics of ecDNA-containing cells under physiological conditions and in the presence of specific selective pressures.

Dynamic Liquid Metal-Microfiber Interlocking Enables Highly Conductive and Strain-insensitive Metastructured Fibers for Wearable Electronics.

Stretchable fibers with high conductivity are vital components for smart textiles and wearable electronics. However, embedding solid conductive materials in polymers significantly reduces conductive pathways when stretched, causing a sharp drop in conductivity. Here, a stretchable metastructured fiber with dynamic liquid metal-microfiber interlocking interface is reported to realize highly conductive yet ultrastable conductance.

Prognostic Value of Coronary CT Angiography-Derived Quantitative Flow Ratio in Suspected Coronary Artery Disease.

Background The prognostic value of coronary CT angiography (CTA)-derived quantitative flow ratio (CT-QFR) remains unknown. Purpose To determine the prognostic value of CT-QFR in predicting the long-term outcomes of patients with suspected coronary artery disease (CAD) in comparison with invasive coronary angiography (ICA)/SPECT and to determine the influence of prior percutaneous coronary intervention (PCI) on the prognostic value of CT-QFR. Materials and Methods In this secondary analysis of the prospective international CORE320 study, 379 participants who underwent coronary CTA and SPECT within 60 days before ICA between November 2009 and July 2011 were included for follow-up.

Hydrogel-Based Sensor for the Detection of Iron Ions and Excessive Alerting.

The excessive content of heavy metal ions in water has attracted increasing worldwide attention due to the potential harm to human health and ecological systems. Therefore, it is particularly significant to research and develop a sensitive and straightforward water quality monitoring method. Here, a dual-network designed hydrogel poly(acrylic acid-co--methylolacrylamide)/poly(3,4-ethylenedioxythiophene): polystyrenesulfonate, P(AA-co-N-MA)/PEDOT: PSS, was constructed and used for Fe detection.

The E3 ligase RAD18-mediated ubiquitination of henipavirus matrix protein promotes its nuclear-cytoplasmic trafficking and viral egress.

The nuclear-cytoplasmic trafficking of matrix proteins (M) is essential for henipavirus budding, with M protein ubiquitination playing a pivotal role in this dynamic process. Despite its importance, the intricacies of the M ubiquitination cascade have remained elusive. In this study, we elucidate a novel mechanism by which Nipah virus (NiV), a highly pathogenic henipavirus, utilizes a ubiquitination complex involving the E2 ubiquitin-conjugating enzyme RAD6A and the E3 ubiquitin ligase RAD18 to ubiquitinate the virus's M protein, thereby facilitating its nuclear-cytoplasmic trafficking.

One-Step Digital Light Processing 3D Printing of Robust, Conductive, Shape-Memory Hydrogel for Customizing High-Performance Soft Devices.

Mechanically robust and electrically conductive hydrogels hold significant promise for flexible device applications. However, conventional fabrication methods such as casting or injection molding meet challenges in delivering hydrogel objects with complex geometric structures and multicustomized functionalities. Herein, a 3D printable hydrogel with excellent mechanical properties and electrical conductivity is implemented via a facile one-step preparation strategy.

Non-invasive in vivo sensing of bacterial implant infection using catalytically-optimised gold nanocluster-loaded liposomes for urinary readout.

Staphylococcus aureus is a leading cause of nosocomial implant-associated infections, causing significant morbidity and mortality, underscoring the need for rapid, non-invasive, and cost-effective diagnostics. Here, we optimise the synthesis of renal-clearable gold nanoclusters (AuNCs) for enhanced catalytic activity with the aim of developing a sensitive colourimetric diagnostic for bacterial infection. All-atom molecular dynamics (MD) simulations confirm the stability of glutathione-coated AuNCs and surface access for peroxidase-like activity in complex physiological environments.

Polymerization-Achieved Cyclodextrin Slide-Ring Supramolecular Hydrogel Self-Generating Flexible Electronic Device.

Supramolecular flexible electronic devices are one of the research hotspots due to their application in the fields of chemistry, biology, and materials. Herein, we reported a slide-ring supramolecular flexible electronic device, which is constructed by acrylamide (AAm), acrylic acid (AA), carboxymethyl-α-cyclodextrin (CM-α-CD), PEG diacrylate (PEGDA), and calcium chloride via the photoinitiated polymerization, displaying not only the mechanical force-responded self-generation but also the human-computer information transfer. As compared with the polymer hydrogel, the addition of α-CD polypseudorotaxane to the hydrogel has notably enhanced both the tensile length and the tensile toughness, making it more suitable for flexible electronic device applications.