Observation of Le Sage gravity analog in complex plasma.

Fragmentation of a suspension of micron-sized plastic microparticles and their contraction into dense globules was experimentally obtained in a gas discharge plasma, when the plasma density was deliberately and abruptly increased. The globules took up spherical shapes 0.14-1.

[The standardization of technique of detection of blood cells cytokine production ex vivo].

The standardized technique to detect the blood cells cytokine production ex vivo was developed. The complex of mitogens of phytohemagglutinin P, phytohemagglutinin M, lipopolysaccharide, concanavalin A is applied to provide the mitogen activation. This approach permits to activate the different types of immunocompetent cells and to induce the secretion of cytokine representative series.

Rapid method for measurement of acetylcholinesterase activity.

Fluorescence intensity of thioflavin T fluorogenic label increased significantly as a result of formation of enzyme-inhibitory complex with acetylcholinesterase of human blood erythrocytes. Thioflavin T is a reversible inhibitor, selectively reacting with acetylcholinesterase. Thioflavin T fluorescence intensity is proportional to acetylcholinesterase activity for the studied interval of enzyme activities.

Gephyrin alterations due to protein accumulation stress are reduced by the lysosomal modulator Z-Phe-Ala-diazomethylketone.

Inhibitory neurotransmission is important for brain function and requires specific transmitter receptors that are organized in synaptic domains. Gephyrin is a cytoskeletal organization protein that binds tubulin and plays an important role in clustering and organizing select inhibitory neurotransmitter receptors. Here, we tested if gephyrin is altered by protein accumulation stress that is common in age-related neurodegenerative disorders.

Lysosomal dysfunction produces distinct alterations in synaptic alpha-amino-3-hydroxy-5-methylisoxazolepropionic acid and N-methyl-D-aspartate receptor currents in hippocampus.

The early processes that lead to synaptic dysfunction during aging are not clearly understood. Dysregulation of alpha-amino-3-hydroxy-5-methylisoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptors may cause age-related cognitive decline. Using hippocampal slice cultures exhibiting lysosomal dysfunction, an early marker of brain aging that is linked to protein accumulation, we identified alterations to AMPA and NMDA receptor-mediated synaptic currents.