A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts.

Methylotrophic yeasts such as and (sin. and , respectively) are commonly used in basic research and biotechnological applications, frequently those requiring genome modifications. However, the CRISPR-Cas9 genome editing approaches reported for these species so far are relatively complex and laborious.

Motivations for adolescent offending and truancy from school: retrospective interviews with adults recently released from a custodial prison sentence in England.

This qualitative study considers the development of adolescent offending and examines a range of potential causes rooted in the issues of truancy, peer pressure, and educational and parental disengagement. Ten adult offenders recently released from prison were accessed through a probation service in the North West of England. Participants ( age = 35.

Phospholipase Superfamily: Structure, Functions, and Biotechnological Applications.

Enzymes of the phospholipase superfamily are involved in lipid metabolism, as well as regulation of membrane composition, cell signaling, and inflammation. This review provides an insight into the structure, functional properties, and biotechnological application of phospholipase A2 and phospholipases in general.

Expression, purification and biophysical characterization of recombinant phospholipase PLA2 overproduced in .

The main purpose of this work was to develop new protocols for high yield purification of secretory phospholipase A2 (PLA2) and to investigate its biophysical properties. We have used a expression system for PLA2 expression and two-stage chromatography for its purification. The biophysical properties of PLA2 were investigated by circular dichroism.

[Modification of Cytotoxic Lymphocytes with T Cell Receptor Specific for Minor Histocompatibility Antigen ACC-1Y].

Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is the only curative therapy for hematopoietic malignancies. The graft-derived donor lymphocytes are capable of eliminating the residual recipient malignant cells in the course of allogeneic immune response, thus decreasing the chances of a relapse of the disease. Foreign peptides of the recipient presented by the MHC molecules are able to elicit the immune response immunologically.