Publications by authors named "S Y Beh"
Mitochondrial electron transport chain (ETC) complexes partition between free complexes and quaternary assemblies known as supercomplexes (SCs). However, the physiological requirement for SCs and the mechanisms regulating their formation remain controversial. Here, we show that genetic perturbations in mammalian ETC complex III (CIII) biogenesis stimulate the formation of a specialized extra-large SC (SC-XL) with a structure of I+III, resolved at 3.
View Article and Find Full Text PDFThe determination of RNA integrity is a critical quality assessment tool for gene expression studies where the experiment's success is highly dependent on the sample quality. Since its introduction in 1999, the gold standard in the scientific community has been the Agilent 2100 Bioanalyzer's RNA integrity number (RIN), which uses a 1-10 value system, from 1 being the most degraded, to 10 being the most intact. In 2015, Agilent launched 4200 TapeStation's RIN equivalent, and reported a strong correlation of r of 0.
View Article and Find Full Text PDFDetermining RNA integrity is a critical quality assessment tool for gene expression studies where the experiment's success is highly dependent on sample quality. Since its introduction in 1999, the gold standard in the scientific community has been the Agilent 2100 Bioanalyzer's RNA Integrity Number (RIN) which uses a 1-10 value system with 1 being the most degraded to 10 being the most intact. In 2015, Agilent launched the 4200 Tapestation's RIN equivalent and reported a strong correlation of r of 0.
View Article and Find Full Text PDFArticle Synopsis
- This chapter outlines methods developed over the past 20 years to isolate microglia from postmortem human brain tissue, including both gray and white matter.
- The technique allows for the simultaneous isolation of vascular cells along with glial cells, enabling comprehensive studies on multiple cell types from the same donor.
- This innovative approach is particularly valuable for -omics research, as it may reveal unique or common molecular characteristics between glial and vascular cells from individual brains.
Article Synopsis
- Nucleotide-binding oligomerization domain (NLR) proteins are key players in the body's immune response to infections, particularly in endothelial cells (ECs) that act as a barrier against pathogens in the bloodstream.
- Research shows that human ECs can activate both NLRP1 and CARD8 inflammasomes in response to a specific inhibitor (VbP) and that the Coxsackie virus B3 (CVB3) primarily activates CARD8, which is crucial for triggering inflammation and cell death.
- Deleting CARD8 from ECs and human heart cells significantly reduces CVB3-induced inflammation and virus spread, highlighting CARD8's important role in the endothelial immune response against viral infections.