Single cell transcriptomic analysis of SGLT2 expression supports an indirect or off-target role for the cardioprotective benefits of empagliflozin in heart failure.

Sodium-glucose cotransporter 2 inhibitors (SGLT2i), such as empagliflozin, have shown remarkable benefits in reducing cardiovascular events and mortality in patients with heart failure irrespective of diabetes. Because of the magnitude of the benefits and broad application in both heart failure with reduced and preserved ejection fraction, there have been concerted efforts to identify a mechanism for the observed benefits. One hypothesis is that SGLT2i act directly on the heart.

Analysis of anti-cancer and anti-inflammatory activity in Typhonium flagelliforme rhizome extract by induction of apoptosis: An in-vitro study.

Plants are major source of bioactive compounds. Typhonium flagelliforme is an important medicinal plant that has various health benefits and crucial properties including analgesic activity, antimicrobial activity, anti-inflammatory activity and anticancer activity. This study aims to determine the anticancer and anti-inflammatory potential in aqueous, acetone, ethanolic, and methanolic extracts of Typhonium flagelliforme against various cell lines using various molecular techniques.

Deciphering the Withania somnifera alkaloids potential for cure of neurodegenerative disease: an in-silico study.

This study investigates the interaction of alkaloids of Withania somnifera with Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), an enzyme implicated in the pathology of various neurodegenerative diseases. Initially, the Withania somnifera phytochemicals were optimised then molecular dockings were performed. The molecular docking results identified key interactions between these alkaloids and active sites of the enzyme.

Cell and transcriptomic diversity of infrapatellar fat pad during knee osteoarthritis.

Objectives: In this study, we employ a multiomic approach to identify major cell types and subsets, and their transcriptomic profiles within the infrapatellar fat pad (IFP), and to determine differences in the IFP based on knee osteoarthritis (KOA), sex and obesity status.

Methods: Single-nucleus RNA sequencing of 82 924 nuclei from 21 IFPs (n=6 healthy control and n=15 KOA donors), spatial transcriptomics and bioinformatic analyses were used to identify contributions of the IFP to KOA. We mapped cell subclusters from other white adipose tissues using publicly available literature.

A Rare Case of Idiopathic Megacolon and Megarectum.

Idiopathic megacolon and megarectum are rare clinical conditions characterized by irreversible dilation of the colon and rectum without an identifiable organic cause. The underlying pathophysiology remains poorly understood, though hypotheses suggest abnormalities in the enteric nervous system or smooth muscle dysfunction. These conditions present significant diagnostic and therapeutic challenges, especially in cases refractory to conservative treatment.