Time-resolved single molecule localization microscopy (TR-SMLM) with a 2 × 2 pixel fiber optic array camera was combined with time-correlated single photon counting (TCSPC) to obtain super-resolved fluorescence lifetime images of individual Cy3 dye molecules and individual colloidal CdSe/CdS/ZnS core/shell/shell semiconductor quantum dots (QDs). The characteristic blinking and bleaching behavior of the Cy3 and the blinking behavior of the QD emitters were used as distinguishing optical characteristics to isolate them and determine their centroid locations with spatial resolution below the optical diffraction limit. TCSPC was used to characterize the fluorescence lifetime and intensity corresponding to each emitter location.
View Article and Find Full Text PDFWe propose a variational wave function to represent quantum skyrmions as bosonic operators. The operator faithfully reproduces two fundamental features of quantum skyrmions: their classical magnetic order and a "quantum cloud" of local spin-flip excitations. Using exact numerical simulations of the ground states of a 2D chiral magnetic model, we find two regions in the single-skyrmion state diagram distinguished by their leading quantum corrections.
View Article and Find Full Text PDFIntroduction: Patient engagement in research aims to foster meaningful partnerships, integrating patient experiences into the research process. This paper describes the development of a Terms of Reference (ToR) to support these meaningful partnerships. While engagement improves data collection and empowerment, ineffective engagement can lead to negative outcomes.
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