Publications by authors named "S Veiga"
Measuring virus in biofluids is complicated by confounding biomolecules coisolated with viral nucleic acids. To address this, we developed an affinity-based microfluidic device for specific capture of intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Our approach used an engineered angiotensin-converting enzyme 2 to capture intact virus from plasma and other complex biofluids.
View Article and Find Full Text PDFThe isolation of VCAM-1 endothelial cell-derived extracellular vesicles using microfluidics.
Extracell Vesicles Circ Nucl Acids
February 2024
Vascular cell adhesion molecule-1 (VCAM-1) endothelial cell-derived extracellular vesicles (EC-EVs) are augmented in cardiovascular disease, where they can signal the deployment of immune cells from the splenic reserve. Endothelial cells in culture activated with pro-inflammatory tumor necrosis factor-α (TNF-a) also release VCAM-1 EC-EVs. However, isolating VCAM-1 EC-EVs from conditioned cell culture media for subsequent in-depth analysis remains challenging.
View Article and Find Full Text PDFA Novel P-III Metalloproteinase from Venom Degrades Extracellular Matrix Proteins, Inhibits Platelet Aggregation, and Disrupts Endothelial Cell Adhesion via α5β1 Integrin Receptors to Arginine-Glycine-Aspartic Acid (RGD)-Containing Molecules.
Toxins (Basel)
November 2024
Article Synopsis
- Viperid snake venoms contain metalloproteinases like barnettlysin-III (Bar-III), which cause bleeding and disrupt blood clotting and tissue integrity in victims.
- Bar-III, a specific type of metalloproteinase, has been characterized in terms of its properties, including its activity being enhanced by calcium and inhibited by zinc, and its ability to degrade important blood and tissue proteins.
- The study also highlights Bar-III’s effects on platelets and endothelial cells, suggesting that it affects platelet aggregation and cell adhesion, providing insights that could lead to new treatments for snakebite envenomation.
Article Synopsis
- - The study focuses on the pacing strategies and race tactics of elite open water swimmers, emphasizing the impact of enhanced data collection during the 2022 World Championships.
- - Researchers analyzed data from 116 swimmers, looking at average velocity, stroke rate, and positions at 17 timing points throughout a 10 km race, revealing that successful swimmers had less conservative pacing compared to past events.
- - Findings indicate a shift in race strategies, with notable variations in pacing and more aggressive finishes, challenging earlier assumptions based on less precise race data.
Induction of ectosome formation by binding of phospholipases D from Loxosceles venoms to endothelial cell surface: Mechanism of interaction.
Biochim Biophys Acta Mol Cell Biol Lipids
November 2024
Members of the phospholipase D (PLD) superfamily found in Loxosceles spider venoms are potent toxins with inflammatory and necrotizing activities. They degrade phospholipids in cell membranes, generating bioactive molecules that activate skin cells. These skin cells, in turn, activate leukocytes involved in dermonecrosis, characterized by aseptic coagulative necrosis.
View Article and Find Full Text PDF