Publications by authors named "S Vageesh Ayyar"
Aging of hematopoietic stem cells (HSCs) is implicated in various aging phenotypes, including immune dysfunction, anemia, and malignancies. The role of HSC proliferation in driving these aging phenotypes, particularly under stress conditions, remains unclear. Therefore, we induced forced replications of HSCs in vivo by a cyclical treatment with low-dose fluorouracil (5FU) and examined the impact on HSC aging.
View Article and Find Full Text PDFArticle Synopsis
- - The text discusses hemophagocytic lymphohistiocytosis (HLH), a severe condition often triggered by a cytokine storm, which can lead to death, particularly in the presence of sepsis that masks early HLH signs.
- - A case study is presented where a patient showed persistent symptoms like high fever and confusion, ultimately being diagnosed with HLH after complications such as deep vein thrombus and renal failure despite treatments.
- - Despite aggressive treatment for HLH, including antineoplastics and corticosteroids due to associated T-cell lymphoma, the patient’s condition deteriorated, leading to shock and eventually cardiac arrest.
sinusitis is an opportunistic fungal infection that is difficult to treat due to its inherent resistance to many antifungal agents. Infections may cause both localized or disseminated disease usually in skin and soft tissues. Immunocompetent persons are typically unaffected and disseminated disease occurs in immunocompromised hosts.
View Article and Find Full Text PDFArticle Synopsis
- Short telomeres are a key characteristic of telomere biology disorders (TBDs) like dyskeratosis congenita (DC), which currently lack effective cures and can lead to bone marrow failure.
- Researchers studied how NAD supplementation, specifically using the precursor nicotinamide riboside (NR), affects health in mice with critically short telomeres.
- Results showed that NR not only improved body weight and telomere integrity but also reduced inflammation and other health issues associated with telomere impairment, hinting at NAD supplementation as a potential treatment option.
Single-cell gel electrophoresis (SCGE or Comet assay) and the Fast Halo assay, also known as the Halo assay, are powerful tools to generate DNA damage measurements with single-cell resolution. Though these techniques are prone to have variability, they can be robust tools for quantifying DNA damage when planned and executed carefully. Here, we present both assays and highlight each technique's advantages and challenges in measuring DNA damage in cells with limiting cell number, such as hematopoietic stem cells (HSCs).
View Article and Find Full Text PDF