Metagenome-validated combined amplicon sequencing and text mining-based annotations for simultaneous profiling of bacteria and fungi: vaginal microbiota and mycobiota in healthy women.

Background: Amplicon sequencing of kingdom-specific tags such as 16S rRNA gene for bacteria and internal transcribed spacer (ITS) region for fungi are widely used for investigating microbial communities. So far most human studies have focused on bacteria while studies on host-associated fungi in health and disease have only recently started to accumulate. To enable cost-effective parallel analysis of bacterial and fungal communities in human and environmental samples, we developed a method where 16S rRNA gene and ITS1 amplicons were pooled together for a single Illumina MiSeq or HiSeq run and analysed after primer-based segregation.

Breastfeeding practices among immigrants living in Finland: Results from the FinChildren survey.

Background: Breastfeeding is a cornerstone of child health and survival as it provides crucial, non-replaceable nourishment necessary for infant's growth and development. Immigration has been shown to influence breastfeeding particularly among immigrants from low- and middle-income countries. Our aim was to examine breastfeeding practices and sociodemographic characteristics of Somali-, Arabic-, and Russian-speaking in comparison with Finnish-speaking mothers.

Environmental Factors in Type 1 Diabetes.

The contribution of environmental factors to the pathogenesis of type 1 diabetes is considered substantial, but their identification has turned out to be challenging. Large prospective studies are crucial for reliable identification of environmental risk and protective factors. However, only few large prospective birth cohort studies have been carried out.

Associations between dietary fibers and gut microbiome composition in the EDIA longitudinal infant cohort.

Background: The infant gut microbiome undergoes rapid changes in the first year of life, supporting normal development and long-term health. Although diet shapes this process, the role of fibers in complementary foods on gut microbiome maturation is poorly understood.

Objectives: We explored how the transition from human milk to fibers in complementary foods shapes the taxonomic and functional maturation of the gut microbiome within the first year of life.