Anaerobic oxidation of methane coupled with sulphate reduction: high concentration of methanotrophic archaea might be responsible for low stable isotope fractionation factors in methane.

The changes in CH and CHH during sulphate-dependent anaerobic oxidation of methane (AOM) were described using dynamic modelling. The batch sulphate-dependent AOM at the nearly linear dynamics of methane oxidation with different enriched cultures originating from three marine sediments was simulated. The traditional Rayleigh equation for carbon and hydrogen stable isotopes in methane was derived from the basic dynamic isotope equation.

Using kinetic isotope effect to evaluate the significance of the sequential and parallel steps: formation of microbial consortium during reversible anaerobic methane oxidation coupled with sulfate reduction.

The purpose of this study was to describe the dynamics of anaerobic oxidation of methane (AOM) coupled with sulfate reduction (SR) using experimental data from a continuous incubation experiments published earlier in order to show that formation of consortia of anaerobic archaea (ANME) and Desulfosarcina-like bacteria (DSS) may have a significant effect on sulfur isotope fractionation. The dynamic simulation of reversible AOM by ANME coupled with SR by DSS was performed. This simulation took into account biomass growth and fractionation of stable isotopes of sulfur.

Modelling the specific pathway of CH and CO formation using carbon isotope fractionation: an example for a boreal mesotrophic fen.

We described mathematically the process of peat methanization in a boreal mesotrophic fen. Gaseous and dissolved CH and CO as well as their δC signatures were considered in the dynamic equations for incubation bottles. In accordance with the model, acetate, H, and CO were produced during cellulose hydrolysis and acidogenesis.

Dynamic changes of carbon isotope apparent fractionation factor to describe transition to syntrophic acetate oxidation during cellulose and acetate methanization.

To identify predominant metabolic pathway for cellulose methanization new equations that take into account dynamics of 13C are added to the basic model of cellulose methanization. The correct stoichiometry of hydrolysis, acidogenesis, acetogenesis and methanogenesis steps including biomass is considered. Using experimental data by Laukenmann et al.

Non-linear dynamics of stable carbon and hydrogen isotope signatures based on a biological kinetic model of aerobic enzymatic methane oxidation.

The non-linear dynamics of stable carbon and hydrogen isotope signatures during methane oxidation by the methanotrophic bacteria Methylosinus sporium strain 5 (NCIMB 11126) and Methylocaldum gracile strain 14 L (NCIMB 11912) under copper-rich (8.9 µM Cu(2+)), copper-limited (0.3 µM Cu(2+)) or copper-regular (1.