Development of novel isothermal-based DNA amplification assay for detection of pig tissues in adulterated meat.

For the first time, we describe an innovative polymerase spiral reaction (PSR) assay for the rapid, simple, and accurate detection of pig tissues or pork in adulterated meat including heat-treated and processed ones. The PSR assay specifically targeting the mitochondrial cytochrome b () gene of the pig was successfully optimized permitting assay results in 65 min time. The developed detection method was 100% specific amplifying only the gene and displaying negative results with all the tested non-pork meats.

Organocatalysis with carbon nitrides.

Carbon nitrides, a distinguished class of metal-free catalytic materials, have presented a good potential for chemical transformations and are expected to become prominent materials for organocatalysis. This is largely possible due to their low cost, exceptional thermal and chemical stability, non-toxicity, ease of functionalization, porosity development, etc. Especially, the carbon nitrides with increased porosity and nitrogen contents are more versatile than their bulk counterparts for catalysis.

from Farm to Table: Isolation, Characterization, and Antimicrobial Resistance of from Commercial Broiler Supply Chain and Its Environment.

Antimicrobial resistance (AMR) in poultry production chain is one of the major food safety concerns due to indiscriminate usage of antibiotics and the presence of pathogens such as which causes infections in various stages of production. In the present study, 182 samples were collected from commercial broiler supply chain, viz., three hatcheries ( = 29), three commercial broiler farms (CBF; = 99), and three retail meat shops (RMS; = 54), and used for isolation and identification of using three different selective agar media and a selective enrichment medium followed by PCR confirmation targeting the gene.