[Characteristics of the NAD-dependent alpha-glycerolphosphate dehydrogenase isoenzymes from vertebrate and human muscles according to polyacrylamide gel electrophoretic and isoelectric focusing data].

Electrophoresis and isoelectric focusing in polyacrylamide gel were used to demonstrate the presence of 5 molecular forms of alpha-glycerophosphate dehydrogenase (E. C. I.

[Identification of multiple molecular forms of lactate dehydrogenase during isoelectric focusing with ampholines in polyacrylamide gel thin layer].

Multiple molecular forms of lactate dehydrogenase fractionated by isoelectric focusing with ampholines in the polyacrylamide gel thin layer were identified in the supernatant fluid of the myocardium and liver homogenate of the intact rats and rabbits. Differential detection of the enzyme fractions was based on differences in their substrate dependences and sensitivity to specific inhibitors. Simultaneously the analysis was made for the partially purified preparations of lactate dehydrogenase five isoenzymes from the same sources obtained by means of the preparative electrophoresis in polyacrylamide gel and ion-exchange adsorption on DEAE-Sephadex A-50.