Recombinant glycoprotein G analog for determination of specific immunoglobulins to herpes simplex virus type 2 by ELISA.

In order to the detection of type-specific IgG to herpes simplex virus type 2 (HSV-2) in human serum or plasma the recombinant analog of HSV-2 glycoprotein G (gG2) was created. To construct an expression vector the DNA fragment with a sequence identical to immunodominant regions of HSV-2 gG2 was cloned into modified vector pET28a containing of the glutation-S-transferase sequence (pET28-GST). Escherichia coli BL21 (DE3) were transformed with the recombinant plasmid.

[Protein kinases of PKD family as a potential object of translational research in oncology].

Recent scientific research demonstrates that protein kinases of PKD family affect biological features of normal and malignant cells. Differential expression of PKD genes was found in tumors of different histogenesis. These protein kinases could be activated by growth factors, antigen stimulation, and oxidative stress, the processes that usually can be observed during tumor progression.

[The role of PKD family protein kinases in the regulation of protein post-translational modification].

The new family of serine/threonine protein kinases D (PKD) belongs to Ca2+/calmodulin-dependent protein kinase group. Here we review with the role of PKDs in the regulation of post-translational modification of cellular proteins. PKDs directly phosphorylate a number of cell signaling molecules, moreover PKDs indirectly regulate histone acetylation and glycosylation of proteins.

Immunohistochemical studies of protein kinase D (PKD) 2 expression in malignant human lymphomas.

Aim: To study the PKD2 expression, autophosphorylation and localization in reactive lymph nodes and tumors of lymphoid tissues.

Materials And Methods: Specific antibodies, which recognize PKD1/2 or PKD2 and autophosphorylated PKD1/2, were used for immunohistochemical and biochemical studies of tonsils, reactive lymph nodes, tumor samples of non-Hodgkin's lymphoma (NHL) and Hodgkin's lymphoma (HL).

Results: Immunohistochemical and biochemical analysis of PKD1 and PKD2 expression showed PKD2 expression in tonsils, reactive lymph nodes and tumor tissues from patients with NHL and HL.

The adaptor protein SH2D1A regulates signaling through CD150 (SLAM) in B cells.

The CD150 receptor is expressed on activated T and B lymphocytes, dendritic cells, and monocytes. A TxYxxV/I motif in the CD150 cytoplasmic tail can bind different SH2-containing molecules, including tyrosine and inositol phosphatases, Src family kinases, and adaptor molecules. To analyze CD150-initiated signal transduction pathways, we used DT40 B-cell sublines deficient in these molecules.