In search of pathogenic Leptospira species in Myotis and other vesper bats, Russia.

Various bat species worldwide have been identified as Leptospira carriers, especially in tropical regions. In this study, we investigated the infection of Vespertilionidae bats by pathogenic Leptospira in north-west Russia. Out of 264 bats from 13 species, the urine of 24 specimens tested positive according to a polymerase chain reaction test.

Generation and characterization of two induced pluripotent stem cell lines (ICGi052-A and ICGi052-B) from a patient with frontotemporal dementia with parkinsonism-17 associated with the pathological variant c.2013T>G in the MAPT gene.

Frontotemporal dementia with parkinsonism-17 is a neurodegenerative disease characterised by pathological aggregation of the tau protein with the formation of neurofibrillary tangles and subsequent neuronal death. The inherited form of frontotemporal dementia can be caused by mutations in several genes, including the MAPT gene on chromosome 17, which encodes the tau protein. As there are currently no medically approved treatments for frontotemporal dementia, there is an urgent need for research using in vitro cell models to understand the molecular genetic mechanisms that lead to the development of the disease, to identify targets for therapeutic intervention and to test potential drugs to prevent neuronal death.

The Generation of Genetically Engineered Human Induced Pluripotent Stem Cells Overexpressing IFN-β for Future Experimental and Clinically Oriented Studies.

Induced pluripotent stem cells (iPSCs) can be generated from various adult cells, genetically modified and differentiated into diverse cell populations. Type I interferons (IFN-Is) have multiple immunotherapeutic applications; however, their systemic administration can lead to severe adverse outcomes. One way of overcoming the limitation is to introduce cells able to enter the site of pathology and to produce IFN-Is locally.

Fast nonlinear Fourier transform algorithm for reconstruction of optical data from nonlinear spectra of the Manakov system.

We propose a high-precision algorithm for solving the three-component Gelfand-Levitan-Marchenko equations (GLME) associated with the Manakov system, which describes the behavior of light waves through the optical fibers. The algorithm generalizes the high-order generalized Toeplitz inner-bordering method for solving the two-component GLME associated with the nonlinear Schrödinger equation. Numerical experiments have shown that the proposed algorithm makes it possible to increase the accuracy of solving the GLME associated with Manakov system up to the sixth order.

CHK1 inhibitor SRA737 is active in PARP inhibitor resistant and amplified ovarian cancer.

High-grade serous ovarian cancers (HGSOCs) with homologous recombination deficiency (HRD) are initially responsive to poly (ADP-ribose) polymerase inhibitors (PARPi), but resistance ultimately emerges. HGSOC with amplification ( ) are associated with resistance to PARPi and platinum treatments. High replication stress in HRD and HGSOC leads to increased reliance on checkpoint kinase 1 (CHK1), a key regulator of cell cycle progression and the replication stress response.