[Protective effect of alpha-tocopherol on the Ca2+-transport system of the sarcoplasmic reticulum membranes in hypercholesterolemia].

The effect of antioxidant--alpha-tocopherol--on Ca2+-transporting system in sarcoplasmic reticulum (SR) of the rabbit skeletal muscles was studied in hypercholesterolemia (HC). alpha-tocopherol administration to animals with HC produced a break on the curve of temperature dependence of Ca-ATPase activity at about 20 degrees C, that disappeared in HC, increased the rate of "rapid" SH-group binding by thiol reagents, and normalized the level of unsaturated fatty acids in SR membranes without altering phospholipid content. It is suggested that the damage of Ca-ATPase in HC is mainly due to activation of lipid peroxidation.

[Endogenous phospholipase hydrolysis in the cerebral cortex during the development of epileptic activity].

The development of epileptic activity in the rat brain cortex is attended by activation of endogenous phospholipase hydrolysis. The development of primary-generalized epileptic activity induced by intramuscular injection of bemegride increased the content of free fatty acids in the brain tissue from 14 +/- 3 micrograms per mg protein in the control to 35 +/- 4 micrograms/mg. The appearance of focal cortical epileptic activity after penicillin application also provoked an increase in the content of free fatty acids in the brain tissue withdrawn from the hyperactive focus (from 40 +/- 5 micrograms/mg in the control to 75 +/- 10 micrograms/mg).

[Effect of nicotinamide on focal and generalized epileptic activity in the cerebral cortex].

Intraperitoneal injection of nicotinamide to rats inhibits epileptic activity induced by penicillin application in the animals' brain cortex. It has been found in experimental primary-generalized epileptic activity induced by bemegride that preliminary injection of nicotinamide increased the latency of the emergence of the first epileptic seizures. Addition of nicotinamide to synaptosomal suspension inhibited accumulation in it of the products of lipid peroxidation.