Publications by authors named "S V Apalko"
The course of COVID-19 is highly variable, with genetics playing a significant role. Through large-scale genetic association studies, a link between single nucleotide polymorphisms and disease susceptibility and severity was established. However, individual single nucleotide polymorphisms identified thus far have shown modest effects, indicating a polygenic nature of this trait, and individually have limited predictive performance.
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- tRNA-derived fragments (tRFs) serve both as biomarkers and signaling molecules in various disorders, with their expression patterns differing among blood cell types and changing with physiological conditions, such as during COVID-19.
- The research compared tRF expression in different blood cells (erythrocytes, monocytes, lymphocytes, etc.) from both healthy donors and severe COVID-19 patients using fluorescence sorting and next-generation sequencing.
- The study found significant changes in tRF patterns in multiple cell types during severe COVID-19, indicating that distinct blood cells respond differently to cytokine storms, which may involve regulation through nucleotide modifications and RNA enzyme activity alterations.
Non-coding RNA expression has shown to have cell type-specificity. The regulatory characteristics of these molecules are impacted by changes in their expression levels. We performed next-generation sequencing and examined small RNA-seq data obtained from 6 different types of blood cells separated by fluorescence-activated cell sorting of severe COVID-19 patients and healthy control donors.
View Article and Find Full Text PDFSevere course of COVID-19 is largely determined by hyperactivation of the immune system, or cytokine storm, in which immune cells (lymphocytes, monocytes, etc.) play a major role. Using low-voltage scanning electron microscopy, we studied the morphology of lymphocytes and monocytes during cytokine storm.
View Article and Find Full Text PDFSevere COVID-19 alters the biochemical and morphological characteristics of blood cells in a wide variety of ways. To date, however, the vast majority of research has been devoted to the study of leukocytes, while erythrocyte morphological changes have received significantly less attention. The aim of this research was to identify erythrocyte morphology abnormalities that occur in COVID-19, compare the number of different poikilocyte types, and measure erythrocyte sizes to provide data on size dispersion.
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