Network pharmacology and animal experiments revealed the protective effects of Guilong prescription on chronic prostatitis and its possible mechanisms.

Objective: To investigate the protective effects of Guilong prescription (, GL) on chronic prostatitis (CP) and unravel the underlying mechanisms of its pharmacological effects.

Methods: The composition of GL was determined linear ion trap/electrostatic field orbital trap tandem high-resolution mass spectrometry, and the identified compounds were performed network pharmacological analysis to predict possible pathways of the effects of GL on CP. A CP rat model was established by carrageenan, and rats were randomly assigned into a Control group, Sham group, CP group, GL low dose (3.