Publications by authors named "S Tuschick"
Article Synopsis
- The study examined the expression of components of the endothelin (ET) system specifically in Purkinje neurons and Bergmann glial cells using a combination of patch-clamp recordings and multiplex RT-PCR.
- Out of 25 Purkinje neurons tested, a high percentage expressed ECE-1 (84%) and ECE-2 (68%) mRNA, but no endothelin or ETA receptor mRNA was detected.
- In contrast, Bergmann glial cells showed a predominant expression of ETB receptor mRNA (68%), along with lower levels of ETA receptor mRNA (16%) and ECE mRNA expressions.
The endothelin (ET) isoforms ET-1, ET-2 and ET-3 applied at 100 nM triggered a transient increase in [Ca2+]i in Bergmann glial cells in cerebellar slices acutely isolated from 20-25 day-old mice. The intracellular calcium concentration ([Ca2+]i) was monitored using Fura-2-based [Ca2+]i microfluorimetry. The ET-triggered [Ca2+]i transients were mimicked by ETB receptor agonist BQ-3020 and were inhibited by ETB receptor antagonist BQ-788.
View Article and Find Full Text PDFThe presence of adrenergic and histaminergic receptors in Bergmann glial cells from cerebellar slices from mice aged 20-25 days was determined using fura-2 Ca2+ microfluorimetry. To measure the cytoplasmic concentration of Ca2+ ([Ca2+]i), either individual cells were loaded with the Ca2+-sensitive probe fura-2 using the whole-cell patch-clamp technique or slices were incubated with a membrane permeable form of the dye (fura-2/AM) and the microfluorimetric system was focused on individual cells. The monoamines adrenalin and noradrenalin (0.
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