Prevalence of hepatitis G virus and its strain variant, the GB agent, in blood donations and their transmission to recipients.

Background: Hepatitis G virus (HGV) and its strain variant, the GB agent (GBV-C) are independent isolates of a recently identified non-A through -E hepatitis virus. Prevalence in United States volunteer blood donors is 1.5 to 1.

Patterns of mycobacterium avium culture and PCR positivity in immunodeficient HIV-infected patients: progression from localized to systematic disease, German Aids Study Group (GASG/IDKF).

Our aim was to establish the frequency and the longitudinal pattern of MAC culture positivity in late stage HIV-infected patients. Two other aims were to analyse risk factors for progression from localized to systemic disease and the value of PCR diagnosis using blood specimens. A total of 107 patients were recruited to be followed for 32 weeks.

[Prevalence of hepatitis G virus genome in blood donors].

The relevance of the GB virus C/hepatitis G virus (GBV-C/HGV) in blood banking results from its high prevalence in blood donors and the fact that it is present at a very high percentage (18%) in polytransfused and hemophiliac patients. Since there is no assay available for serological testing, we developed a sensitive PCR utilizing newly designed NS3 primers to investigate the prevalence in blood donors in Hessia. Testing 1,143 accepted blood donors with alaninaminotransferase (ALT) concentrations < 45 U/l we found 15 (1.

Cloning and molecular characterization of the secY genes from Bacillus licheniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family.

SecY is a central component of the export machinery that mediates the translocation of secretory proteins across the plasma membrane of Escherichia coli. We have cloned and sequenced the secY genes from Bacillus licheniformis and Staphylococcus carnosus. The deduced amino acid sequences are highly homologous to those of other known SecY polypeptides, all having the potential to form 10 transmembrane segments.