Synergistic Interaction of 5-HT and 5-HT Receptors in Cytoplasmic Ca Regulation in Human Umbilical Vein Endothelial Cells: Possible Involvement in Pathologies.

The aim of this work was to explore the involvement of 5-HT and 5-HT receptors (5-HTR and 5-HTR) in the regulation of free cytoplasmic calcium concentration ([Ca]) in human umbilical vein endothelial cells (HUVEC). We have shown by quantitative PCR analysis, that 5-HTR and 5-HTR mRNAs levels are almost equal in HUVEC. Immunofluorescent staining demonstrated, that 5-HTR and 5-HTR are expressed both in plasma membrane and inside the cells.

[On the issue of separate designation of the pre-Descemet's layer in the structure of the cornea].

The five-layer structure of the cornea has been generally accepted in anatomy for the last 70 years. Since the beginning of 2000, publications about lamellar keratoplasty have appeared, in which during the operation it was necessary to isolate the Descemet's membrane (DM). Several options for the formation of a big bubble have been described; in the most frequently used type, the presence of remnants of thin stromal tissue on the isolated DM were found.

SARS-CoV-2 Receptors and Their Involvement in Cell Infection.

The new coronavirus infection (COVID-19) pandemic caused by SARS-CoV-2 has many times surpassed the epidemics caused by SARS-CoV and MERS-CoV. The reason for this was the presence of sites in the protein sequence of SARS-CoV-2 that provide interaction with a broader range of receptor proteins on the host cell surface. In this review, we consider both already known receptors common to SARS-CoV and SARS-CoV-2 and new receptors specific to SARS-CoV-2.

[Optical properties of the cornea after different modifications of penetrating keratoplasty].

Purpose: Comparative analysis of optical properties of the cornea after different modifications of penetrating keratoplasty.

Material And Methods: The study included 56 patients (56 eyes) divided into three study groups according to the surgery technique. Group I included 17 patients (17 eyes) after one-piece mushroom keratoplasty, group II - 21 patients (21 eyes) after two-piece mushroom keratoplasty and group III - 18 patients (18 eyes) after penetrating keratoplasty.

[Comparative evaluation of the results of one- and two-piece mushroom keratoplasty].

Purpose: To compare and evaluate the outcomes of one- and two-piece mushroom keratoplasty configurations for various corneal pathologies.

Material And Methods: A total of 32 patients (32 eyes) with corneal perforations, descemetocele and deep corneal opacities underwent surgery. Depending on the surgery technique, the patients were divided into 2 groups: group I (17 eyes) underwent manual one-piece mushroom keratoplasty according to our own technique; group II (15 eyes) underwent modified two-piece microkeratome-assisted mushroom keratoplasty according to the technique by M.