Publications by authors named "S Trapmann"
Objectives: In this paper, we describe the steps followed for the development of a certified reference material for immunoglobulin G antibodies against β2-glycoprotein I (also known as apolipoprotein H). These steps include processing of the material, commutability, the impact of dilution, the appropriate reconstitution conditions, homogeneity and stability during transport and storage.
Methods: We analysed 69 clinical samples from patients suffering from antiphospholipid syndrome with several commercial enzyme-linked immunosorbent assays (ELISA) purchased from in vitro diagnostic manufacturers.
Article Synopsis
- Certified reference materials for amyloid beta (Aβ) were created to improve the accuracy of diagnostic tests for Aβ concentration in human cerebrospinal fluid.
- Three different certified reference materials (ERM-DA480/IFCC, ERM-DA481/IFCC, ERM-DA482/IFCC) were prepared and characterized, with mass concentrations of Aβ measured using advanced isotope dilution mass spectrometry.
- Following the re-calibration of various immunoassays using these reference materials, the discrepancy in test results was significantly reduced, demonstrating improved consistency across different testing methods.
Digital PCR has become the emerging technique for the sequence-specific detection and quantification of nucleic acids for various applications. During the past years, numerous reports on the development of new digital PCR methods have been published. Maturation of these developments into reliable analytical methods suitable for diagnostic or other routine testing purposes requires their validation for the intended use.
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- Digital PCR offers a highly accurate method for quantifying nucleic acid fragments, especially in viral RNA analysis.
- The study investigates how factors like primer/probe chemistry, PCR target, duplexing, and template type affect results in reverse transcription digital PCR (RT-dPCR) using the influenza A virus.
- Notably, the choice of primer/probe chemistry and template type significantly influenced RNA quantification, underlining the necessity for method optimization to ensure precision in molecular diagnostics for viral pathogens.