Real time monitoring and quantification of uptake carbon nanodots in eukaryotic cells.

The real time monitoring and quantification of the concentration of highly fluorescent nitrogen-doped carbon nanodots (C-dots) in eukaryotic Tobacco bright yellow-2 (BY-2) plant cells was investigated by fluorescence and confocal microscopy. The quantitative measurement of their fluorescent emission intensity was possible because of the high photo-resistance, good water solubility and the absence of fading effect of the nanoparticles, which is frequent occurred problem of the conventional organic dyes. The microscopic analysis revealed that C-dots entered generally into the cells through endocytosis and caused negligible cytotoxicity.

Visualization of the native shape of bodipy-labeled DNA in Escherichia coli by correlative microscopy.

The native shape and intracellular distribution of newly synthesized DNA was visualized by correlative (light and electron) microscopy in ice embedded whole cells of Escherichia coli. For that purpose, the commercially available modified nucleoside triphosphate named BODIPY® FL-14-dUTP was enzymatically incorporated in vivo into the genome of E. coli mutant K12 strain, which cannot synthesize thymine.

The impact of the extent of surgical resection on survival of gastric cancer patients.

Objective: The aim of this study was to examine the significance of the extent of gastric resection on the postoperative and overall gastric cancer survival.

Background: Resection with clean margins (4 cm or more) is widely accepted as the standard-ized goal for radical treatment of gastric cancer according to current guidelines, while the type of resection (subtotal or total) is still a matter of debate.

Patients And Methods: The study included 155 patients diagnosed and treated in the Department of Surgery, Aleksandrovska University Hospital between January 2005 and December 2014.

Electron microscopic visualization of complementary labeled DNA with platinum-containing guanine derivative.

The object of the present report is to provide a method for a visualization of DNA in TEM by complementary labeling of cytosine with guanine derivative, which contains platinum as contrast-enhanced heavy element. The stretched single-chain DNA was obtained by modifying double-stranded DNA. The labeling method comprises the following steps: (i) stretching and adsorption of DNA on the support film of an electron microscope grid (the hydrophobic carbon film holding negative charged DNA); (ii) complementary labeling of the cytosine bases from the stretched single-stranded DNA pieces on the support film with platinum containing guanine derivative to form base-specific hydrogen bond; and (iii) producing a magnified image of the base-specific labeled DNA.

[Inguinal endometriosis: two case reports and literature review].

Endometriosis is a common gynecological disease that is characterized by the presence of endometrial glands and stroma outside the uterus. Ectopic endometrial tissue can be localized in all organs of the human body, but it affects most often organs in the pelvis. The localization in the inguinal canal is extremely rare - 0.