Age-dependent susceptibility to severe disease with primary exposure to Plasmodium falciparum.

This study investigated the incidence of severe disease following primary exposure to Plasmodium falciparum by nonimmune children and adults in Irian Jaya, Indonesia. Four months after arrival, the cross-sectional prevalence of P. falciparum was 72%, and the monthly cumulative incidence of clinical diagnoses of malaria was 81%.

Cultivation of sexually mature Brugia malayi in vitro.

Sexually mature male and female Brugia malayi were developed from third stage larvae after 60 days in the in vitro culture system described by Franke and others in 1987 (Am J Trop Med Hyg 37: 370-375). Between 75 and 100 days in culture, many worms produced living microfilariae. Each gravid female produced 200-1,500 microfilariae/day.

The pathology of lymphoreticular and genital tissues of silvered leaf monkeys (Presbytis cristata) experimentally infected with Wuchereria bancrofti.

The discovery of the silvered leaf monkey, Presbytis cristata, as a suitable experimental host for the human filarial parasite, Wuchereria bancrofti, opened the door for major advances in our understanding of the disease caused by this parasite. To study the pathogenesis of bancroftian filariasis in this model, 15 adult P. cristata which had been experimentally infected with 250 infective third-stage larvae of the parasite were examined.

Use of Indonesian non-human primate erythrocytes for measles hemagglutination inhibition serology.

The use of erythrocytes (RBC) from Presbytis cristata, P. melalophus and Macaca fascicularis for measles hemagglutination inhibition serologies is presented. These primates can provide a ready supply of RBC for diagnostic reagent use in areas of the world where the African green monkey or rhesus macaque are not available.