Effects of refeeding with low- or high-carbohydrate diets on intermediary carbohydrate metabolism in juvenile and adult Nile tilapia.

Generally, energy expenditure and compensation according to food deprivation and refeeding often occur along the life cycle of farmed-raised fish. Fasting and refeeding are also hypothesised to modulate carbohydrate metabolism particularly for herbivorous and/or omnivorous. This study aims to investigate the effects of short-term fasting and subsequent refeeding with high or low-carbohydrate diets on the intermediary carbohydrate metabolism of juvenile and adult Nile tilapia (Oreochromis niloticus) which is known to be a good user of carbohydrate as an energy source.

Cryopreservation of the whole testes of Asian sea bass (Lates calcarifer) and its effects on apoptosis, germ cell-specific gene expression, germ cell transplantability, and DNA methylation.

Cryopreservation of spermatogonia could be a useful tool to preserve the genetic resources of fish, which could be further restored via germ cell transplantation. In this study, the protocol for the cryopreservation of the spermatogonia of Asian sea bass (Lates calcarifer), an economically important fishery resource in the Indo-West Pacific, was optimised. The impact of the cryopreservation technique on cell viability and apoptosis, expression of several genes related to immature germ cell markers, transplantability in allogeneic recipients, and global DNA methylation was evaluated.