Gene-editing in patient and humanized-mice primary muscle stem cells rescues dysferlin expression in dysferlin-deficient muscular dystrophy.

Dystrophy-associated fer-1-like protein (dysferlin) conducts plasma membrane repair. Mutations in the DYSF gene cause a panoply of genetic muscular dystrophies. We targeted a frequent loss-of-function, DYSF exon 44, founder frameshift mutation with mRNA-mediated delivery of SpCas9 in combination with a mutation-specific sgRNA to primary muscle stem cells from two homozygous patients.

Single-neuron spiking variability in hippocampus dynamically tracks sensory content during memory formation in humans.

During memory formation, the hippocampus is presumed to represent the content of stimuli, but how it does so is unknown. Using computational modelling and human single-neuron recordings, we show that the more precisely hippocampal spiking variability tracks the composite features of each individual stimulus, the better those stimuli are later remembered. We propose that moment-to-moment spiking variability may provide a new window into how the hippocampus constructs memories from the building blocks of our sensory world.

Status of Serum Magnesium among Patients with Chronic Kidney Disease.

Chronic kidney disease (CKD) is one of the largest public health issues and the major cause of death worldwide. Due to an increase in the incidence of systemic diseases like diabetes and hypertension, which are the two main causes of CKD and kidney damage, the prevalence of CKD is rising globally. Chronic Kidney Disease results in mineral disorders as well as profound lipid disorders due to improper balancing of the mineral & hormone levels in the body.

Effect of Serum Vitamin-D in Patients with Type 2 Diabetes Mellitus.

Type 2 diabetes, formerly known as adult-onset diabetes, is a form of diabetes mellitus that is characterized by high blood sugar, insulin resistance and relative lack of insulin. This cross sectional study was carried out in the Department of Biochemistry, Mymensingh Medical College and in collaboration with the Department of Endocrinology, Mymensingh Medical College Hospital, Mymensingh from July 2021 to June 2022. A total of 100 subjects were included in this study.

Status of Serum Iron in Patients with Chronic Obstructive Pulmonary Disease.

Chronic obstructive pulmonary disease (COPD) remains a major public health problem with relatively high prevalence rates worldwide (5.0-13.0%).