Improving identification of low abundance and hydrophobic proteins using fluoroalcohol mediated supramolecular biphasic systems with quaternary ammonium salts.

In this study, a newly discovered Supramolecular Biphasic System (S-BPS) was used in bottom-up proteomics of the Saccharomyces cerevisiae strain of yeast. We took advantage of S-BPS in bottom-up proteomics of this strain of yeast as the protein sample, while the results were compared to routinely used solubilizing reagents, such as urea, and sodium dodecyl sulfate (SDS). With the S-BPS, we identified 3043 proteins as compared to 2653 proteins that were identified in the control system.

Improved Protein Coverage in Bottom-Up Proteomes Analysis Using Fluoroalcohol-Mediated Supramolecular Biphasic Systems With Mixed Amphiphiles for Sample Extraction, Fractionation, and Enrichment.

A new class of supramolecular biphasic systems containing fluoroalcohol-induced coacervates (FAiC) provides concomitant fractionation of complex protein mixtures, high solubilizing power for extraction of various types of proteins, especially those with high hydrophobicity (such as membrane proteins), and enrichment of low-abundance proteins. Subsequently, the use of FAiC biphasic systems (BPS) in the bottom-up proteomics workflow resulted in significantly higher coverage for the whole proteome, various subproteomes, especially those embedded or associated with membranes, post-translationally modified proteins, and low-abundance proteins (LAPs) as compared to the conventional methodologies. In this work, we used a new type of FAiC-BPS composed of mixed amphiphiles, a zwitterionic surfactant 3-(,-dimethylmyristyl ammonia) propane sulfonate (DMMAPS), a quaternary ammonium salt (QUATS), and hexafluoroisopropanol (HFIP) as the coacervator for extraction, fractionation, and enrichment of yeast proteome in bottom-up proteomics.

Effects of intra-yolk-sac injection of dextrose and albumin on performance, jejunum morphology, liver and pectoral muscle glycogen and some serum metabolites of broilers.

This study carried out to investigate the effects of intra-yolk-sac injection (IYSI) of some solutions including 1 ml of distilled water, dextrose 20% and albumin 20% on hatch percentage, performance traits, jejunum morphology, glycogen content of liver and breast and serum metabolites in broilers (Ross 308). Fertile eggs were injected into the yolk sac at day 8 of incubation period. Results showed that hatchability, absolute body weight (BW), feed intake (FI) and feed conversion ratio (FCR) at day 7 and 14 of growing period were not different among treatments, but in comparison with control group, BW and FCR were numerally better by IYSI of albumin.

Effects of dietary supplementation of waste date's vinegar on performance and improvement of digestive tract in broiler chicks.

Two hundred 1-day-old commercial broilers (Ross 308) were used to determine the effects of diets supplementation with waste date's vinegar (WDV) on the growth and performance of digestive tract over a 42-days growing period. Chicks were randomly allocated to one of five experimental diets supplementing as 0 (control), 1, 2, and 3% of WDV and 2% industrial vinegar (IV). Broilers and their feed consumptions were weighed at the trial beginning and days 10, 21, 35 and 42 of experimental period.