[EXPRESS IDENTIFICATION OF POSITIVE BLOOD CULTURES USING DIRECT MALDI-TOF MASS SPECTROMETRY].

Objective: To evaluate the effectiveness of direct identification of pathogens of bacteremia by direct matrix assisted laser desorption ionization time-flight mass spectrometry (mALDI-TOF) compared to routine method.

Material And Methods: A prospective study included 211 positive blood cultures obtained from 116 patients (106 adults and 10 children, aged from 2 weeks to 77 years old in the ICU after open heart surgery. Incubation was carried out under aerobic vials with a sorbent for antibiotics Analyzer BacT/ALERT 3D 120 (bioMerieux, France) in parallel with the primary sieving blood cultures on solid nutrient media with subsequent identification of pure cultures using MALDI-TOF mass spectrometry analyzer Vitek MS, bioMerieux, France routine method), after appropriate sample preparation we carried out a direct (without screening) MALDI-TOF mass spectrometric study of monocomponental blood cultures (n = 201).

Prognostic value of sCD14-ST (presepsin) in cardiac surgery.

Introduction: Prediction of complications and mortality after cardiac surgery is an important aspect of timely correction of these conditions. One possibility in this case is the use of biomarkers and some prognostic scores.

Aim Of The Study: To study the prognostic value of presepsin (PSP) as a predictor of postoperative complications development in cardiosurgical patients.

[Procalcitonin as a predictor of bacteremia in postoperative cardiosurgery patients].

Purpose Of The Study: To define diagnostics ability of procalcitonin (PCT) test for prediction of bacteremia of different etiology in cardio-surgical patients.

Materials And Methods: 10158 pairs of blood examples from cardio-surgical patients in early postoperative period were studied from 2007 to 2012. Results of PCT test were compared with blood cultures.

[SCD14-ST (presepsin) level monitoring in cardiac surgical patients during perioperative period].

Unlabelled: Purpose of the study was to define prognostic ability of presepsin (sCD14-ST) as a predictor of complications in cardiac surgical patients during perioperative period.

Methods: Patients operated for acquired heart valvular diseases with cardiopulmonary bypass were involved in the study (n = 51, age 58 +/- 11 years). Following parameters were studied; demographic data, duration of cardiopulmonary bypass, time of aorta clamping, severity-of-disease by APACHE II scale before surgery, on 1st, 2nd, 3rd and 6th day after surgery, routine clinical laboratory data and sCD14-ST.