Influence of chronic carbon disulphide intoxication on the development of experimental atherosclerosis in rats.

Rats fed on atherogenic diets containing 2% cholesterol and 0.5% cholic acid with or without 0.15% thiouracil were exposed to carbon disulphide (CS2) vapours (1 mg/l of air), five hours a day, six days a week for 6-10 months.

Preliminary project of the standard minimal scope and methods of testing acute toxicity of industrial chemical substances.

The standard minimal scope of experimental toxicity evaluation of industrial chemicals has been proposed. On the basis of literature and own experience the authors propose that the minimal scope of toxicity testing should include determination of lethal doses and evaluation of morphological alterations of inner organs in acute experiments, as well as assessment of eye and skin irritation and contact sensitization. The main lines of uniform procedures are presented.

[Lipid changes in the blood serum and arterial wall in rabbits chronically exposed to CS2].

In the studies carried out lipids content in blood serum and aorta wall as well as morphological changes in coronary vessels of CS2 exposed rats have been evaluated. The 10-month-exposure to CS2 at a concentration of 0,8--0,9 mg/l has been found to result in: (1) increased concentration of total and free cholesterol, cholesterol esters, triglycerides and phospholipids in blood serum; (2) increase in total cholesterol and significant rise in the level of cholesterol esters in the aorta wall; (3) a relative increase in serum VLDL, LDL and decrease in HDL fraction. Histological estimation has demonstrated in serial sections of hearts of the CS2 exposed animals thickened walls of left coronary artery branches with no lipid deposits.

[Biological and histological changes induced by cement dust].

In animal experiments pneumoconiogenic properties of metallurgic and Portland cement dust, produced in Poland, were investigated. Experimental pneumoconiosis was developed by intratracheal single administration, to white rats, of 50 mg of dust suspended in 0.6 ml of NaCl physiological solution.