Pattern of Lyme arthritis in Europe: report of 14 cases.

Fourteen cases of Lyme arthritis are reported. The most frequent picture was that of oligoarthritis appearing in that part of the leg where the cutaneous or neurological complications, or both, of Lyme disease had developed before the arthritis. In most cases recovery followed a single 10 day course of intravenous (IV) penicillin therapy.

Rapid double-sandwich enzyme-linked immunosorbent assay for detection of human immunoglobulin M anti-Toxoplasma gondii antibodies.

The double-sandwich enzyme-linked immunosorbent assay has been compared with the indirect fluorescence assay for the detection of immunoglobulin M antibodies against Toxoplasma gondii in humans. Incubation times have been shortened, permitting the test to be completed within 2 h. The double-sandwich enzyme-linked immunosorbent assay is confirmed to be more sensitive and more specific than the immunofluorescence assay.

ELISA using whole Legionella pneumophila cell as antigen. Comparison between monovalent and polyvalent antigens for the serodiagnosis of human legionellosis.

An indirect enzyme-linked immunosorbent assay (ELISA) using the six serogroups of whole L. pneumophila bound to microtitre plate wells is described for the serodiagnosis of legionellosis. Comparative studies using monovalent antigen indicated a high correlation between ELISA and indirect immunofluorescence antibody (IFA) tests (r = 0.

Serodiagnosis of human G and M immunoglobulins to Toxoplasma gondii by ELISA using whole tachyzoites as antigens: a comparative study with the indirect haemagglutination (IHA) and immunofluorescence (IFA) tests.

An indirect ELISA using whole tachyzoites of Toxoplasma gondii fixed onto the bottom of microtiter plate wells is described for detection of specific G and M anti-toxoplasma antibodies. ELISA results were compared with those of IFA and IHA tests. Similarity of antigens (cell surface) involved in ELISA and IFA permits high correlation between the two tests (r = 0.

Use of whole Streptococcus pneumoniae cells as a solid phase sorbent for C-reactive protein measurement by ELISA.

Monolayers of pneumococcus (serotype 27) on flat bottom polystyrene microtiter plates were used as a solid phase sorbent for the determination of C-reactive protein (CRP) by ELISA. After binding to the monolayer, CRP was quantified with peroxidase conjugated rabbit anti-human CRP immunoglobulin. The method is sensitive (5 micrograms/ml), rapid (less than 2 h) and correlates well with a laser nephelometric assay (r = 0.